72 kD
20 mM Tris-HCl、1 mM CaCl2、50% Glycerol、pH 7.4 @ 25°C
Store at -25 ~ -15℃ for 1 years
[1] Kushnirov VV, Dergalev AA, Alexandrov AI. Proteinase K resistant cores of prions and amyloids. Prion. 2020 Dec;14(1):11-19.
[2] Xu C , Battig A , Schartel B ,et al.Investigation of the Thermal Stability of Proteinase K for the Melt Processing of Poly(l-lactide)[J].Biomacromolecules, 2022, 23(11):4841-4850.
Proteinase K is a powerful proteolytic enzyme with high specific activity and is a key reagent for DNA extraction. The enzyme is active in a wide pH range (4~12.5) and at high temperature (50~70°C), and is used for the isolation of plasmid or genomic DNA and RNA. In DNA extraction, the main function is to enzymatically dissociate the histones bound to nucleic acids, so that the DNA is free in the solution, and then extracted by different methods to remove impurities and collect the DNA. chelating agents such as EDTA or descaling agents such as SDS can not inactivate the enzyme.
Storage Solution:20 mM Tris-HCl、1mM CaCl2、50% glycerol (pH 7.4 @ 25°C)
Add the specified amount of Proteinase K storage solution according to the nucleic acid extraction instructions. Commonly used working concentrations are 50-100 µg/mL
Please avoid repeated freeze-thaw cycles.
M: marker
Lane 1: 2μg PK is reduced and thermally denatured;
Lane 2: 2μg PK under reducing condition;
Lane 3: 2μg PK is non-reduced and thermally denatured;
Lanes 4: 2μg PK under non-reducing condition;
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