Met1-Cys624, with C-terminal His
94kDa (Reducing)
20mM Tris, 500mM NaCl, 3mM DTT, 10% glycerol, pH 7.4.
· 12 months from date of receipt, lyophilized powder stored at -20 to -80℃.
· 3 months, -20 to -80℃ under sterile conditions after reconstitution.
· 1 week, 2 to 8℃ under sterile conditions after reconstitution.
· Please avoid repeated freeze-thaw cycles.
Mol Cell Biol.2005 Jan;25(1):162-71.
Chem Res Toxicol. 2008 Mar;21(3):705-10. Epub 2008 Feb 6.
Biol Chem.2010 Jul 16;285(29):22576-91. Epub 2010 May 7.
Biochemistry. 2004 Sep 28;43(38):12113-22.
KEAP1 acts as a key sensor of oxidative and electrophilic stress: in normal conditions, the BCR(KEAP1)complex mediates ubiquitination and degradation of NFE2L2/NRF2, a transcription factor regulating expression of many cytoprotective genes. In response to oxidative stress, different electrophile metabolites trigger non-enzymatic covalent modifications of highly reactive cysteine residues in KEAP1, leading to inactivate the ubiquitin ligase activity of the BCR(KEAP1) complex, promoting NFE2L2/NRF2 nuclear accumulation and expression of phase II detoxifying enzymes. In response to selective autophagy, KEAP1 is sequestered in inclusion bodies following its interaction with SQSTM1/p62, leading to inactivation of the BCR(KEAP1) complex and activation of NFE2L2/NRF2. The BCR(KEAP1)complex also mediates ubiquitination of SQSTM1/p62, increasing SQSTM1/p62 sequestering activity and degradation .It also targets BPTF and PGAM5 for ubiquitination and degradation by the proteasome.
1μg (R: reducing condition, N: non-reducing condition).
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