RNase H-抗体-北京谱研生物科技有限公司

产品规格

分子别名

Ribonuclease HI、RNase HI、Ribonuclease H、RNase H
表达序列

/
分子量

19 kD
纯度

＞95% by SDS-PAGE
活性

5U/μl
标记

Unconjugated
标签

His Tag
性状

Liquid
缓冲体系

50 mM KCl、10 mM Tris-HCl、0.1 mM EDTA、1 mM DTT、200 µg/ml BSA 50% Glycerol（pH 7.4 @ 25°C）
溶解方法

/
储存条件

Store at -25 ~ -15℃ for 2 years
文献引用

[1] Chan S H , Whipple J M , Dai N ,et al.RNase H-based analysis of synthetic mRNA 5' cap incorporation[J].RNA (New York, N.Y.), 2022, 28(8):1144-1155.
[2] Ilina T V , Brosenitsch T , Sluis-Cremer N ,et al.Retroviral RNase H: Structure, mechanism, and inhibition[J]. 2021.

背景介绍

Endonuclease that specifically degrades the RNA of RNA-DNA hybrids. RNase H participates in DNA replication; it helps to specify the origin of genomic replication by suppressing initiation at origins other than the oriC locus; along with the 5'-3' exonuclease of pol1, it removes RNA primers from the Okazaki fragments of lagging strand synthesis; and it defines the origin of replication for ColE1-type plasmids by specific cleavage of an RNA preprimer. Involved in production of retron derived msDNA (a branched RNA linked by a 2',5'-phosphodiester bond to a single-stranded DNA).

产品组分

Storage Solution: 5U/μl RNase H、50 mM KCl、10 mM Tris-HCl、0.1 mM EDTA、1 mM DTT、200 µg/ml BSA 50% Glycerol（pH 7.4 @ 25°C） 10*Reaction Buffer: 500 mM Tris-HCl、750 mM KCl、30 mM MgCl2、100mM DTT(pH 8.3 @ 25°C)

操作步骤

Set-up a typical reaction as follows 1）Add the following components in sequence 2）Incubate at 37°C for 20 minutes This reaction can be scaled up according to experimental needs.

注意事项

1、Both metal ion chelating agent and sulfhydryl sealer can inhibit RNase H activity 2、Heating at 65℃ can be inactivated for 10min

酶活定义

One unit is the amount of enzyme required to hydrolyze 1 nmol nucleotide from a 20 pmol fluorescent-labeled 50 bp RNA-DNA hybridization chain at 37℃ for 20 minutes in a 50 µl reaction system.

RNase H