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IL-12 Protein,Mouse

IL-12 Protein,Mouse

货号: UA040218
价格: 1323
规格: 10μg
介绍: -
其他: -
产品规格

  • 物种

    Mouse

  • 分子别名

    IL-12a Protein, Mouse; IL-12b Protein, Mouse; IL-12p35 Protein, Mouse; IL-12p40 Protein, Mouse; IL12; IL-12; IL-12, subunit p35; IL12A; interleukin 12, p35; CLMF p35; p35; CLMF1; Cytotoxic lymphocyte maturation factor 35 kDa subunit; IL-12 subunit p35; NKSF1; NK cell stimulatory factor chain 1; interleukin-12 alpha chain; interleukin-12 subunit alpha; natural killer cell stimulatory factor 1, 35 kD subunit; p35 Protein, Mouse; p40 Protein, Mouse

  • Accession

    P43432 , P43431

  • 表达序列

    p40: Met23-Ser335; p35: Arg23-Ala215, with C-terminal His Tag

  • 表达宿主

    CHO

  • 分子量

    P40: 35.8 kDa (Reducing)

    P35: 22.5 kDa (Reducing)

  • 纯度

    ＞95% by SDS-PAGE and HPLC

  • 内毒素含量

    <0.1EU/μg

  • 活性

    EC50 < 0.6ng/ml.

  • 标记

    Unconjugated

  • 标签

    His Tag

  • 性状

    Lyophilized Powder

  • 缓冲体系

    PBS, pH7.4.

  • 溶解方法

    Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation.

  • 储存条件

    · 12 months from date of receipt, lyophilized powder stored at -20 to -80℃.
    · 3 months, -20 to -80℃ under sterile conditions after reconstitution.
    · 1 week, 2 to 8℃ under sterile conditions after reconstitution.
    · Please avoid repeated freeze-thaw cycles.

  • 文献引用

    1. Airoldi, I. et al. (2000) Journal of Immunology 165:6880.

    2. Wolf, S.F. et al. (1991) Journal of Immunology 146:3074.

    3. Gearing, D. et al. (1991) Cell 66:9.

    4. Merberg, D. et al. (1992) Immunology Today 13:78.

    5. Gubler, U. et al. (1991) Proc. Natl. Acad. Sci. 88:4143.

  • 稀释度

背景介绍

  • IL-12 is involved in the differentiation of naive T cells into Th1 cells. It is known as a T cell-stimulating factor, which can stimulate the growth and function of T cells. It stimulates the production of interferon-gamma (IFN-γ) and tumor necrosis factor-alpha (TNF-α) from T cells and natural killer (NK) cells, and reduces IL-4 mediated suppression of IFN-γ. T cells that produce IL-12 have a coreceptor, CD30, which is associated with IL-12 activity. IL-12 plays an important role in the activities of natural killer cells and T lymphocytes. IL-12 mediates enhancement of the cytotoxic activity of NK cells and CD8+ cytotoxic T lymphocytes.

  • 生物活性JSON

    • Measured by its ability to enhance IFN-gamma secretion in NK-92 human natural killer lymphoma cells. The EC50 for this effect is less than 0.6ng/ml.

  • 电泳JSON

    • 2μg (R: reducing condition, N: non-reducing condition).

  • 反相高效液相色谱JSON(RP-HPLC)

    • >95% as determined by RP-HPLC.