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Biotinylated B7-H3/CD276 His&Avi Tag Protein, Human

Biotinylated B7-H3/CD276 His&Avi Tag Protein, Human

货号: UA010328
价格: 2800
规格: 25μg
介绍: -
其他: -
产品规格

  • 物种

    Human

  • 分子别名

    B7-H3, CD276;B7H3, B7-H3, CD276 antigen, CD276 molecule, CD276, B7H34Ig-B7-H3, B7-H3B7 homolog 3, Costimulatory molecule

  • Accession

    Q5ZPR3-2

  • 表达序列

    Leu29-Pro245, with C-terminal 10*His&Avi tag LEVQVPEDPVVALVGTDATLCCSFSPEPGFSLAQLNLIWQLTDTKQLVHSFAEGQDQGSAYANRTALFPDLLAQGNASLRLQRVRVADEGSFTCFVSIRDFGSAAVSLQVAAPYSKPSMTLEPNKDLRPGDTVTITCSSYRGYPEAEVFWQDGQGVPLTGNVTTSQMANEQGLFDVHSVLRVVLGANGTYSCLVRNPVLQQDAHGSVTITGQPMTFPHHHHHHHHHHGLNDIFEAQKIEWHE

  • 表达宿主

    HEK293

  • 分子量

    38-48kDa

  • 纯度

    >95% by SDS-PAGE

  • 内毒素含量

    <1EU/μg

  • 标记

    Biotin

  • 标签

    Avi Tag, His Tag

  • 性状

    Lyophilized Powder

  • 缓冲体系

    PBS, pH7.4

  • 溶解方法

    Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation.

  • 储存条件

    · 12 months from date of receipt, lyophilized powder stored at -20 to -80℃.
    · 3 months, -20 to -80℃ under sterile conditions after reconstitution.
    · 1 week, 2 to 8℃ under sterile conditions after reconstitution.
    · Please avoid repeated freeze-thaw cycles.

  • 文献引用

    1、Liu J. et al. (2021) Targeting B7-H3 via chimeric antigen receptor T cells and bispecific killer cell engagers augments antitumor response of cytotoxic lymphocytes. J Hematol Oncol. 14(1): 21.

  • 稀释度

背景介绍

  • B7-h3 (B7 homolog 3 protein), also known as CD276, is an important immune checkpoint molecule in the B7-CD28 family. It is a type I transmembrane glycoprotein consisting of 316 amino acids and contains a putative 28AA signal peptide, a 217AA extracellular region composed of immunoglobulin constant (IgC) and variable (IgV) structures, a transmembrane region, and a 45-amino acid cytoplasmic domain. B7-H3 is a T cell co-suppressor molecule with partial co-stimulatory function. B7-H3 can effectively inhibit the function of T cells and NK cells, and also play a role in bone development. The expression of B7-H3 is low in normal tissues and is found in a variety of malignant tumors, which is closely related to the growth, metastasis, recurrence and poor prognosis of malignant tumors. B7-H3 can down-regulate T-assisted type 1 mediated immune response, inhibit CD4+T cell activation and inhibit cytokine production, and thus may play a role in promoting immune escape of cancer cells.

  • 电泳JSON

    • 1μg (R: reducing conditions, N: non-reducing conditions).

  • ELISA

    • Immobilized Biotinylated B7-H3/CD276 His&Avi Tag, Human (Cat. No. UA010328) at 2 μg/mL on Streptavidin precoated (0.5μg/well) plate, can bind Anti-Human B7-H3 Monoclonal Antibody (Enoblituzumab) with EC50 of 2.50-3.30 ng/ml.