12 months from date of receipt / reconstitution, -20 °C as supplied
| 应用 | 稀释度 |
|---|---|
| WB | 1:1000 |
| IP | 1:50 |
Mediator of RNA polymerase II transcription subunit 4 also known as mediator complex subunit 4 (MED4), a component of Mediator or vitamin D3 receptor-interacting protein complex 36 kDa component (DRIP36) is a protein that in humans is encoded by the MED4 gene. The protein encoded by this gene is a component of the vitamin D receptor-interacting protein (DRIP) complex which functions as a nuclear receptor coactivator. The DRIP complex is capable of activating nuclear receptors in a ligand-dependent manner.
WB result of MED4 Rabbit mAb
Primary antibody: MED4 Rabbit mAb at 1/1000 dilution
Lane 1: HepG2 whole cell lysate 20 µg
Lane 2: K562 whole cell lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 30 kDa
Observed MW: 36 kDa
WB result of MED4 Rabbit mAb
Primary antibody: MED4 Rabbit mAb at 1/1000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 30 kDa
Observed MW: 36 kDa
WB result of MED4 Rabbit mAb
Primary antibody: MED4 Rabbit mAb at 1/1000 dilution
Lane 1: PC-12 whole cell lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 30 kDa
Observed MW: 36 kDa
MED4 Rabbit mAb at 1/50 dilution (1 µg) immunoprecipitating MED4 in 0.4 mg HepG2 whole cell lysate.
Western blot was performed on the immunoprecipitate using MED4 Rabbit mAb at 1/1000 dilution.
Secondary antibody (HRP) for IP was used at 1/400 dilution.
Lane 1: HepG2 whole cell lysate 20 µg (Input)
Lane 2: MED4 Rabbit mAb IP in HepG2 whole cell lysate
Lane 3: Rabbit monoclonal IgG IP in HepG2 whole cell lysate
Predicted MW: 30 kDa
Observed MW: 36 kDa
（This blot was developed with high sensitivity substrate）
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