12 months from date of receipt / reconstitution, 4 °C as supplied.
| 应用 | 稀释度 |
|---|---|
| ICC | 1:500 |
| ICFCM | 1:200 |
Progesterone receptor (PR) is a master regulator in female reproductive tissues that controls developmental processes and proliferation and differentiation during the reproductive cycle and pregnancy. PR also plays a role in progression of endocrine-dependent breast cancer. As a member of the nuclear receptor family of ligand-dependent transcription factors, the main action of PR is to regulate networks of target gene expression in response to binding its cognate steroid hormone, progesterone [PMID: 27380738].
Flow cytometric analysis of 4% PFA fixed 90% methanol permeabilized MDA-MB-231 (Human breast adenocarcinoma epithelial cell, left) / T-47D (Human ductal breast epithelial tumor epithelial cell, right) cells labelling Progesterone Receptor (Alexa Fluor 488 Conjugate) antibody at 1/200 (1 μg) dilution/ (red) compared with a Rabbit monoclonal IgG isotype control (Black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Negative control: MDA-MB-231
ICC shows positive staining in T-47D cells. Anti- Progesterone Receptor (Alex Fluor® 488 Conjugate) antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue).
Negative control: ICC shows negative staining in MDA-MB-231 cells. Anti-Progesterone Receptor (Alex Fluor® 488 Conjugate) antibody was used at 1/500 dilution and incubated overnight at 4°C. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue).
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