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CAIX Recombinant Rabbit mAb (SDT-356-21)

CAIX Recombinant Rabbit mAb (SDT-356-21)

货号: S0B0109
价格: 600
规格: 25ul
介绍: -
其他: -
产品规格
  • 宿主来源

    Rabbit
  • 抗原名称

    CAIX
  • 分子别名

    CA 9, Carbonic anhydrase 9, Carbonate dehydratase IX, Membrane antigen MN homolog
  • 免疫原

    Recombinant Protein
  • 细胞定位

    Cytoplasm, Cell membrane
  • Accession

    Q8VHB5
  • 克隆号

    SDT-356-21
  • 抗体类型

    Recombinant mAb
  • 应用

    IHC-P, WB
  • 反应种属 ?

    Ms, Rt
  • 纯化方式

    Protein A
  • 浓度

    0.5 mg/ml
  • 标签

    N/A
  • 性状

    Liquid
  • 缓冲体系

    PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
  • 储存条件

    12 months from date of receipt / reconstitution, -20 °C as supplied
稀释度
应用 稀释度
WB 1:1000
IHC-P 1:8000
背景介绍
  • Carbonic anhydrase IX has been under intensive investigation as a therapeutic target in cancer. Studies demonstrate that this enzyme has a key role in pH regulation in cancer cells, allowing these cells to adapt to the adverse conditions of the tumour microenviroment. Novel CAIX inhibitors have shown efficacy in both in vitro and in vivo pre-clinical cancer models, adversely affecting cell viability, tumour formation, migration, invasion, and metastatic growth when used alone. In co-treatments, CAIX inhibitors may enhance the effects of anti-angiogenic drugs or chemotherapy agents [PMID: 29439394].

  • 免疫印迹

    • WB result of CAIX Rabbit mAb 
      Primary antibody: CAIX Rabbit mAb at 1/1000 dilution
      Lane 1: mouse stomach lysate 20 µg
      Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 50kDa
      Observed MW: 54kDa

  • 免疫组化

    • IHC shows positive staining in paraffin-embedded mouse stomach. Anti-CAIX antibody was used at 1/8000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • Negative control: IHC shows negative staining in paraffin-embedded mouse liver. Anti-CAIX antibody was used at 1/8000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded rat stomach. Anti-CAIX antibody was used at 1/8000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • Negative control: IHC shows negative staining in paraffin-embedded rat kidney. Anti-CAIX antibody was used at 1/8000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.