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Androgen Receptor Recombinant Rabbit mAb (SDT-R157)

Androgen Receptor Recombinant Rabbit mAb (SDT-R157)

货号: S0B2180
价格: 600
规格: 25μl
介绍: -
其他: -
产品规格

  • 宿主来源

    Rabbit

  • 抗原名称

    Androgen Receptor

  • 分子别名

    AR, Dihydrotestosterone receptor, Nuclear receptor subfamily 3 group C member 4

  • 免疫原

    N/A

  • 细胞定位

    Nucleus

  • Accession

    P10275

  • 克隆号

    SDT-R157

  • 抗体类型

    Recombinant mAb

  • 应用

    ICFCM, IHC-P, ICC, WB

  • 反应种属 ?

    Hu, Ms, Rt

  • 纯化方式

    Protein A

  • 浓度

    0.5 mg/ml

  • 性状

    Liquid

  • 缓冲体系

    PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300

  • 储存条件

    12 months from date of receipt / reconstitution, -20 °C as supplied

稀释度
应用 稀释度
WB 1:1000
IHC-P 1:1000
ICFCM 1:500
ICC 1:500
背景介绍

  • The androgen receptor (AR), ligand-induced transcription factor, is expressed in primary prostate cancer and in metastases. AR regulates multiple cellular events, proliferation, apoptosis, migration, invasion, and differentiation. Its expression in prostate cancer cells is regulated by steroid and peptide hormones [PMID: 24384911]. The elucidation of the structures of the AR DNA binding domain (DBD) and ligand binding domain (LBD) provides a new framework for understanding the functions of this receptor and leads to the development of rational drug design for the treatment of prostate cancer [PMID: 24909511].

  • 免疫印迹

    • WB result of Androgen Receptor Rabbit mAb Primary antibody: Androgen Receptor Rabbit mAb at 1/1000 dilution Lane 1: PC-3 whole cell lysate 20 µg Lane 2: LNCaP whole cell hot lysate with 1% SDS 20 µg Negative control: PC-3 whole cell lysate Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 98kDa Observed MW: 98kDa
    • WB result of Androgen Receptor Rabbit mAb Primary antibody: Androgen Receptor Rabbit mAb at 1/1000 dilution Lane 1: rat testis lysate 20 µg Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 98kDa Observed MW: 98kDa

  • 流式分析

    • Flow cytometric analysis of 4% PFA fixed 90% methanol permeabilized Jurkat (Human T cell leukemia T lymphocyte, left) / LNCAP (Human prostate carcinoma epithelial cell, right) cells labelling Androgen Receptor antibody at 1/500 dilution (0.1 μg) / (red) compared with a Rabbit monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti - Rabbit IgG Alexa Fluor® 488 was used as the secondary antibody. 
      Negative control: Jurkat

  • 免疫组化

    • IHC shows positive staining in paraffin-embedded human pancreatic cancer. Anti-Androgen Receptor antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
    • IHC shows positive staining in paraffin-embedded human breast cancer. Anti-Androgen Receptor antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
    • Negative control: IHC shows negative staining in paraffin-embedded human kidney. Anti-Androgen Receptor antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
    • Negative control: IHC shows negative staining in paraffin-embedded human colon cancer. Anti-Androgen Receptor antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
    • Negative control: IHC shows negative staining in paraffin-embedded human cervical squamous cell carcinoma. Anti-Androgen Receptor antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
    • IHC shows positive staining in paraffin-embedded mouse testis. Anti-Androgen Receptor antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

  • 免疫细胞化学

    • ICC shows positive staining in LnCap cells. Anti-Androgen Receptor antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue).