12 months from date of receipt / reconstitution, -20 °C as supplied
| 应用 | 稀释度 |
|---|---|
| WB | 1:1000-1:5000 |
| IHC-P | 1:200 |
| ICFCM | 1:50 |
| ICC | 1:50 |
| IP | 1:25 |
Beta III-tubulin is frequently overexpressed in human tumors and is associated with resistance to microtubule-targeting agents, tumor aggressiveness, and poor patient outcome [PMID: 34364992IF: 9.7 Q1 ]. In prostate cancer, βIII-tubulin expression is strongly associated with both TMPRSS2: ERG rearrangement, ERG expression and PTEN deletions, three key oncogenetic features of aggressive prostate cancer [PubMed: 24378408]. Also, colorectal cancer is more aggressive in young male patients in whom testosterone elevation and activation of TUBB3 are connected with poor outcome [PubMed: 23637935].
Flow cytometric analysis of 4% PFA fixed 90% methanol permeabilized SH-SY5Y (Human neuroblastoma epithelial cell) cells labelling Beta III tubulin antibody at 1/50 (0.1 μg) dilution / (red) compared with a Rabbit monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti - Rabbit IgG Alexa Fluor® 488 was used as the secondary antibody.
Beta III tubulin Rabbit mAb at 1/25 dilution (0.2 µg) immunoprecipitating Beta III tubulin in 0.2 mg SH-SY5Y whole cell lysate.
Western blot was performed on the immunoprecipitate using Beta III tubulin Rabbit mAb at 1/1000 dilution.
Secondary antibody (HRP) for IP was used at 1/400 dilution.
Lane 1: SH-SY5Y whole cell lysate 5 µg (Input)
Lane 2: Beta III tubulin Rabbit mAb IP in SH-SY5Y whole cell lysate
Lane 3: Rabbit monoclonal IgG IP in SH-SY5Y whole cell lysate
Predicted MW: 50 kDa
Observed MW: 55 kDa
ICC shows positive staining in SH-SY5Y cells. Anti-Beta III tubulin antibody was used at 1/50 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue).
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