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HNF4α Recombinant Rabbit mAb (SDT-373-64)

HNF4α Recombinant Rabbit mAb (SDT-373-64)

货号: S0B2172
价格: 260
规格: 10μl
介绍: -
其他: -
产品规格

  • 宿主来源

    Rabbit

  • 抗原名称

    HNF4α

  • 分子别名

    Hepatocyte nuclear factor 4, NR2A1, HNF-4-alpha, HNF-4, TCF-14

  • 免疫原

    Recombinant Protein

  • 细胞定位

    Nucleus

  • Accession

    P49698

  • 克隆号

    SDT-373-64

  • 抗体类型

    Recombinant mAb

  • 应用

    ICFCM, IHC-P, ICC, WB, IP

  • 反应种属 ?

    Hu, Ms, Rt

  • 纯化方式

    Protein A

  • 浓度

    0.5 mg/ml

  • 性状

    Liquid

  • 缓冲体系

    PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300

  • 储存条件

    12 months from date of receipt / reconstitution, -20 °C as supplied

稀释度
应用 稀释度
WB 1:1000
IHC-P 1:500
ICC 1:500
IP 1:50
ICFCM 1:500
背景介绍

  • HNF4α is a transcription factor that plays an important role in liver morphogenesis and maintenance of proper hepatocyte function in a mature liver. Most liver diseases have been associated with altered HNF4α expression, isoform ratios, and localization. Therefore, many studies have indicated that HNF4α may potentially be a target gene for the regression of fibrosis and cirrhosis [PMID: 35114889].

  • 免疫印迹

    • WB result of HNF4α Rabbit mAb Primary antibody: HNF4α Rabbit mAb at 1/1000 dilution Lane 1: HeLa whole cell lysate 20 µg Lane 2: HepG2 whole cell lysate 20 µg Lane 3: SW480 whole cell lysate 20 µg Lane 4: Caco-2 whole cell lysate 20 µg Negative control: HeLa whole cell lysate Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 53kDa Observed MW: 45~55 kDa

  • 流式分析

    • Flow cytometric analysis of 4% PFA fixed 90% methanol permeabilized HeLa (Left) / HepG2 (Right) cells labelling HNF4α antibody at 1/500 dilution (0.1 μg)/ (Red) compared with a Rabbit monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti-Rabbit IgG Alexa Fluor® 488 was used as the secondary antibody. Negative control: HeLa

  • 免疫沉淀

    • HNF4α Rabbit mAb at 1/50 dilution (1 µg) immunoprecipitating HNF4α in 0.4 mg HepG2 whole cell lysate.
      Western blot was performed on the immunoprecipitate using HNF4α Rabbit mAb at 1/1000 dilution.
      Secondary antibody (HRP) for IP was used at 1/400 dilution.
      Lane 1: HepG2 whole cell lysate 20 µg (Input)
      Lane 2: HNF4α Rabbit mAb IP in HepG2 whole cell lysate
      Lane 3: Rabbit monoclonal IgG IP in HepG2 whole cell lysate
      Predicted MW: 53 kDa
      Observed MW: 45~55 kDa
      Exposure time: 20 s

  • 免疫组化

    • IHC shows positive staining in paraffin-embedded human colon. Anti-HNF4α antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
    • IHC shows positive staining in paraffin-embedded human kidney. Anti-HNF4α antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
    • IHC shows positive staining in paraffin-embedded human colon cancer. Anti-HNF4α antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
    • IHC shows positive staining in paraffin-embedded mouse colon. Anti-HNF4α antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
    • IHC shows positive staining in paraffin-embedded mouse liver. Anti-HNF4α antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
    • IHC shows positive staining in paraffin-embedded rat kidney. Anti-HNF4α antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

  • 免疫细胞化学

    • ICC shows positive staining in HepG2 cells. Anti-HNF4α antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue).
    • Negative control: ICC shows negative staining in HeLa cells. Anti-HNF4α antibody was used at 1/500 dilution and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue).