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SPARC Recombinant Rabbit mAb (SDT-R136)

SPARC Recombinant Rabbit mAb (SDT-R136)

货号: S0B0066
价格: 600
规格: 25μl
介绍: -
其他: -
产品规格

  • 宿主来源

    Rabbit

  • 抗原名称

    SPARC

  • 分子别名

    BM-40, Osteonectin, ON, Secreted protein acidic and rich in cysteine

  • 免疫原

    N/A

  • 细胞定位

    Basement membrane, Secreted, Extracellular matrix

  • Accession

    P09486

  • 克隆号

    SDT-R136

  • 应用

    IHC-P, WB, IP

  • 反应种属 ?

    Hu

  • 纯化方式

    Protein A

  • 浓度

    0.5 mg/ml

  • 性状

    Liquid

  • 缓冲体系

    PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300

  • 储存条件

    12 months from date of receipt / reconstitution, -20 °C as supplied

稀释度
应用 稀释度
WB 1:1000
IHC-P 1:2000
IP 1:50
背景介绍

  • SPARC, also referred to as osteonectin or basement membrane protein 40 (BM-40), is a ~32 kDa calcium-binding matricellular protein [PMID: 22249026, PMID: 25064829]. At one time SPARC was thought to be expressed specifically in mineralized tissues (thus the name osteonectin) but was subsequently demonstrated to have a much wider expression pattern in both mineralized and non-mineralized tissues [PMID: 7034958]. Typically, expression of SPARC is closely aligned with that of fibrillar collagens such as collagen type I. The protein component of the pre-mineralized bone matrix (osteoid) is composed mainly of collagen type I (94%) and a number of noncollagenous proteins.

  • 免疫印迹

    • WB result of SPARC Rabbit mAb Primary antibody: SPARC Rabbit mAb at 1/1000 dilution Lane 1: Jurkat whole cell lysate 20 µg Lane 2: SH-SY5Y whole cell lysate 20 µg Lane 3:A-375 whole cell lysate 20 µg Negative control: Jurkat whole cell lysate Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 35 kDa Observed MW: 37 kDa

  • 免疫沉淀

    • SPARC Rabbit mAb at 1/50 dilution (1 µg) immunoprecipitating SPARC in 0.4 mg A375 whole cell lysate.
      Western blot was performed on the immunoprecipitate using SPARC Rabbit mAb at 1/1000 dilution.
      Secondary antibody (HRP) for IP was used at 1/400 dilution.
      Lane 1: A375 whole cell lysate 20 µg (Input)
      Lane 2: SPARC Rabbit mAb IP in A375 whole cell lysate
      Lane 3: Rabbit monoclonal IgG IP in A375 whole cell lysate
      Predicted MW: 35 kDa
      Observed MW: 37 kDa


  • 免疫组化

    • IHC shows positive staining in paraffin-embedded human tonsil. Anti-SPARC antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
    • IHC shows positive staining in paraffin-embedded human kidney. Anti-SPARC antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
    • IHC shows positive staining in paraffin-embedded human testis. Anti-SPARC antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
    • IHC shows positive staining in paraffin-embedded human breast cancer. Anti-SPARC antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
    • IHC shows positive staining in paraffin-embedded human hepatocellular carcinoma. Anti-SPARC antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
    • IHC shows positive staining in paraffin-embedded human lung squamous cell carcinoma. Anti-SPARC antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.