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Hemoglobin Recombinant Rabbit mAb (SDT-193-71)

Hemoglobin Recombinant Rabbit mAb (SDT-193-71)

货号: S0B0057
价格: 600
规格: 25μl
介绍: -
其他: -
产品规格

  • 宿主来源

    Rabbit

  • 抗原名称

    Hemoglobin

  • 分子别名

    Hb

  • 免疫原

    Native protein

  • 细胞定位

    Cytosol, Blood microparticle

  • Accession

    P69905, P68871

  • 克隆号

    SDT-193-71

  • 抗体类型

    Rabbit mAb

  • 应用

    IHC-P, WB, IP

  • 反应种属 ?

    Hu

  • 纯化方式

    Protein A

  • 浓度

    0.25 mg/ml

  • 性状

    Liquid

  • 缓冲体系

    PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300

  • 储存条件

    12 months from date of receipt / reconstitution, -20 °C as supplied

稀释度
应用 稀释度
WB 1:500
IHC-P 1:5000
IP 1:25
背景介绍

  • The primary function of Hemoglobin (Hb) is to transport oxygen (O2) from the lung to tissues, binding and releasing O2 in a cooperative manner [PMID: 32189307]. Hemoglobin is a tetramer, consisting of two α-subunits (α1 and α2) and two βsubunits (β1 and β2) that are structurally similar and about the same size. The two αβ dimers (named α1β1 and α2β2) are arranged around a 2-fold axis of symmetry resulting in a large central water cavity in the T or unliganded or deoxygenated structure and a narrower cavity in the R or liganded or oxygenated structure.

  • 免疫印迹

    • WB result of Hemoglobin Rabbit mAb Primary antibody: Hemoglobin Rabbit mAb at 1/500 dilution Lane 1: 293T whole cell lysate 20 µg Lane 2: TF-1 whole cell lysate 20 µg Negative control: 293T whole cell lysate Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 13 kDa Observed MW: 13 kDa

  • 免疫沉淀

    • Hemoglobin Rabbit mAb at 1/25 dilution (1µg) immunoprecipitating Hemoglobin in 0.4mg TF-1 whole cell lysate.
      Western blot was performed on the immunoprecipitate using Hemoglobin Rabbit mAb at 1/1000 dilution.
      Secondary antibody (HRP) for IP was used at 1/400 dilution.
      Lane 1 : TF-1 whole cell lysate 20µg(input)
      Lane 2 : Hemoglobin Rabbit mAb IP in TF-1 whole cell lysate
      Lane 3 : Rabbit monoclonal IgG IP in TF-1 whole cell lysate
      Predicted MW: 13 kDa
      Observed MW: 13 kDa

  • 免疫组化

    • IHC shows positive staining in paraffin-embedded human tonsil. Anti-Hemoglobin antibody was used at 1/5000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
    • IHC shows positive staining in paraffin-embedded human placenta. Anti-Hemoglobin antibody was used at 1/5000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
    • IHC shows positive staining in paraffin-embedded human prostate. Anti-Hemoglobin antibody was used at 1/5000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
    • IHC shows positive staining in paraffin-embedded human cardiac muscle. Anti-Hemoglobin antibody was used at 1/5000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
    • IHC shows positive staining in paraffin-embedded human stomach. Anti-Hemoglobin antibody was used at 1/5000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.