PBS pH7.4, containing no preservative
2 to 8 °C for 2 weeks under sterile conditions;
-20 °C for 3 months under sterile conditions;
-80 °C for 24 months under sterile conditions.
Please avoid repeated freeze-thaw cycles.
| 应用 | 稀释度 |
|---|---|
| WB | 1:300 |
This antibody recognizes the same epitope as clone 10F.9G2.
Programmed death-ligand 1 (PD-L1) also known as cluster of differentiation 274 (CD274) or B7 homolog 1 (B7-H1) is a protein that in humans is encoded by the CD274 gene. Programmed death-ligand 1 (PD-L1) is a 40kDa type 1 transmembrane protein that has been speculated to play a major role in suppressing the adaptive arm of immune systems during particular events such as pregnancy, tissue allografts, autoimmune disease and other disease states such as hepatitis. The binding of PD-L1 to the inhibitory checkpoint molecule PD-1 transmits an inhibitory signal based on interaction with phosphatases (SHP-1 or SHP-2) via Immunoreceptor Tyrosine-Based Switch Motif (ITSM). This reduces the proliferation of antigen-specific T-cells in lymph nodes, while simultaneously reducing apoptosis in regulatory T cells (anti-inflammatory, suppressive T cells) - further mediated by a lower regulation of the gene Bcl-2. Upregulation of PD-L1 on immune cells (especially myeloid cells) can also lead to formation of an immunosuppressive environment in a highly localized manner that also allow the cancer cells to proliferate.
PD-L1/B7-H1 Fc Chimera (Mouse recombinant protein) at 0.25 μg/mL can bind PD-L1 Recombinant Rat mAb (10F.9G2) at 200 nm/L.
KD(M)=3.689*10-8
WB result of PD-L1 Rabbit mAb
Primary antibody: PD-L1 Rabbit mAb at 1/300 dilution
Lane 1: PD-L1/B7-H1 Fc Chimera, Mouse (recombinant protein) lysate 1μg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 64~78 kDa
Observed MW: 70 kDa
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