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SMAD4 Recombinant Rabbit mAb (SDT-168-67)

SMAD4 Recombinant Rabbit mAb (SDT-168-67)

货号: S0B0033
价格: 600
规格: 25μl
介绍: -
其他: -
产品规格

  • 宿主来源

    Rabbit

  • 抗原名称

    Smad4

  • 分子别名

    Mothers against decapentaplegic homolog 4; MAD homolog 4; Mothers against DPP homolog 4; Deletion target in pancreatic carcinoma 4; hSMAD4

  • 免疫原

    Synthetic Peptide

  • 细胞定位

    Cytoplasm, Nucleus

  • Accession

    Q13485

  • 克隆号

    SDT-168-67

  • 抗体类型

    Recombinant mAb

  • 抗体同种型

    IgG

  • 应用

    WB, IP

  • 反应种属 ?

    Hu, Ms, Rt

  • 预测反应种属
    (反应种属缩写表)

    Bv, Pg

  • 纯化方式

    Protein A

  • 浓度

    0.5 mg/ml

  • 标记

    Unconjugated

  • 性状

    Liquid

  • 缓冲体系

    PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300

  • 储存条件

    12 months from date of receipt / reconstitution, -20 °C as supplied

稀释度
应用 稀释度 推荐种属
WB 1:1000 Hu, Ms, Rt
IP 1:50 Hu, Ms, Rt
背景介绍

  • SMAD (mothers against decapentaplegic homologs) molecules are the core components in TGF-β signaling pathway. TGF-β binding to its receptor induces phosphorylation and activation of receptor-regulated SMADs (R-SMADs), SMAD2 and SMAD3, which subsequently associate with their partner SMAD4 and translocate from cytoplasm to nucleus. Formation of R-SMAD–SMAD4 complexes is essential in signaling of most TGF-β family members.

  • 免疫印迹

    • WB result of Smad4 Rabbit mAb
      Primary antibody: Smad4 Rabbit mAb at 1/1000 dilution
      Lane 1: SW480 whole cell lysate 20 µg
      Lane 2: HT-29 whole cell lysate 20 µg
      Lane 3: HeLa whole cell lysate 20 µg
      Lane 4: HepG2 whole cell lysate 20 µg
      Lane 5: Jurkat whole cell lysate 20 µg
      Negative control: SW480 whole cell lysate;
      HT-29 whole cell lysate
      Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 70 kDa
      Observed MW: 65 kDa
      Exposure time: 180s

    • WB result of Smad4 Rabbit mAb
      Primary antibody: Smad4 Rabbit mAb at 1/1000 dilution
      Lane 1: NIH/3T3 whole cell lysate 20 µg
      Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 70 kDa
      Observed MW: 65 kDa
      Exposure time: 180s

    • WB result of Smad4 Rabbit mAb
      Primary antibody: Smad4 Rabbit mAb at 1/1000 dilution
      Lane 1: C6 whole cell lysate 20 µg
      Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 70 kDa
      Observed MW: 65 kDa
      Exposure time: 180s

  • 免疫沉淀

    • SMAD4 Rabbit mAb at 1/50 dilution (1 µg) immunoprecipitating SMAD4 in 0.4 mg HeLa whole cell lysate.
      Western blot was performed on the immunoprecipitate using SMAD4 Rabbit mAb at 1/1000 dilution.
      Secondary antibody (HRP) for IP was used at 1/1000 dilution.
      Lane 1: HeLa whole cell lysate 20 µg (Input)
      Lane 2: SMAD4 Rabbit mAb IP in HeLa whole cell lysate
      Lane 3: Rabbit monoclonal IgG IP in HeLa whole cell lysate
      Predicted MW: 70 kDa
      Observed MW: 70 kDa