您现在的位置： 首页   -  产品中心   -  免疫学   -   抗体
GST-π Recombinant Rabbit mAb (SDT-R054)

GST-π Recombinant Rabbit mAb (SDT-R054)

货号: S0B0032
价格: 600
规格: 25μl
介绍: -
其他: -
产品规格

  • 宿主来源

    Rabbit

  • 抗原名称

    GSTP-1

  • 分子别名

    Glutathione S-Transferase Pi, GST-pi, GST class-pi, GSTP1-1

  • 免疫原

    N/A

  • 细胞定位

    Cytoplasm, Nucleus

  • Accession

    P09211

  • 克隆号

    SDT-R054

  • 抗体类型

    Rabbit mAb

  • 应用

    IHC-P, ICC, WB

  • 反应种属 ?

    Hu, Ms, Rt

  • 纯化方式

    Protein A

  • 浓度

    0.25 mg/ml

  • 性状

    Liquid

  • 缓冲体系

    PBS, 40% Glycerol, 0.05%BSA, 0.03% Proclin 300

  • 储存条件

    12 months from date of receipt / reconstitution, -20 °C as supplied

稀释度
应用 稀释度
WB 1:500-1:2500
IHC-P 1:2000
ICC 1:50
背景介绍

  • GST-π is abundantly expressed in some mammalian tissues, particularly those associated with malignancies. While the enzyme can catalyze thioether bond formation between some electrophilic chemicals and GSH, novel non-detoxification functions are now ascribed to it.

  • 免疫印迹

    • WB result of GST-π Rabbit mAb Primary antibody: GST-π Rabbit mAb at 1/2500 dilution Lane 1: LNCaP whole cell lysate 20 µg Lane 2: K-562 whole cell lysate 20 µg Negative control: LNCaP whole cell lysate Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 23 kDa Observed MW: 23 kDa
    • WB result of GST-π Rabbit mAb Primary antibody: GST-π Rabbit mAb at 1/2500 dilution Lane 1: LNCaP whole cell lysate 20 µg Lane 2: Jurkat whole cell lysate 20 µg Negative control: LNCaP whole cell lysate Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 23 kDa Observed MW: 23 kDa
    • WB result of GST-π Rabbit mAb Primary antibody: GST-π Rabbit mAb at 1/500 dilution Lane 1: mouse liver lysate 20 µg Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 23 kDa Observed MW: 23 kDa
    • WB result of GST-π Rabbit mAb Primary antibody: GST-π Rabbit mAb at 1/500 dilution Lane 1: rat kidney lysate 20 µg Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 23 kDa Observed MW: 23 kDa

  • 免疫组化

    • IHC shows positive staining in paraffin-embedded human kidney. Anti-GST-π antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
    • IHC shows positive staining in paraffin-embedded human lung. Anti-GST-π antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
    • IHC shows positive staining in paraffin-embedded human lung squamous cell carcinoma. Anti-GST-π antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
    • IHC shows positive staining in paraffin-embedded mouse liver. Anti-GST-π antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
    • IHC shows positive staining in paraffin-embedded rat kidney. Anti-GST-π antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

  • 免疫细胞化学

    • ICC shows positive staining in K562 cells. Anti-GST-π antibody was used at 1/50 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (red).