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S100A13 Recombinant Rabbit mAb (SDT-R040)

S100A13 Recombinant Rabbit mAb (SDT-R040)

货号: S0B0028
价格: 600
规格: 25μl
介绍: -
其他: -
产品规格
  • 宿主来源

    Rabbit
  • 抗原名称

    S100A13
  • 分子别名

    S100 calcium-binding protein A13
  • 免疫原

    N/A
  • 细胞定位

    Cytoplasm, Secreted
  • Accession

    Q99584
  • 克隆号

    SDT-R040
  • 抗体类型

    Rabbit mAb
  • 应用

    IHC-P, WB
  • 反应种属 ?

    Hu
  • 纯化方式

    Protein A
  • 浓度

    0.0625 mg/ml
  • 性状

    Liquid
  • 缓冲体系

    PBS, 40% Glycerol, 0.05%BSA, 0.03% Proclin 300
  • 储存条件

    12 months from date of receipt / reconstitution, -20 °C as supplied
稀释度
应用 稀释度
IHC-P 1:1000
WB 1:1000
背景介绍
  • S100A13 is a 11 kDa member of the S100 (soluble in 100% saturated ammonium sulfate) family of vertebrate EF-Hand CA ion binding protein. S100A13 is widely expressed in various types of tissue, and high protein expression is detected in thyroid filter cells, testicular Leydig cells, and brain -specific cells. S100A13 can regulate the secretion of FGF1 and IL1α, and has potential to become a symbol for the production of tumors and endometrial vascular. CK19 and S100A13 in cPTC are significantly increased, indicating that S100A13 may be regarded as a new candidate PTC biomarker.

  • 免疫印迹

    • WB result of S100A13 Rabbit mAb
      Primary antibody: S100A13 Rabbit mAb at 1/1000 dilution
      Lane 1: Daudi whole cell lysate 20 µg
      Lane 2: HeLa whole cell lysate 20 µg
      Lane 3: HUVEC whole cell lysate 20 µg
      Negative control: Daudi whole cell lysate
      Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 11 kDa
      Observed MW: 13 kDa

  • 免疫组化

    • IHC shows positive staining in paraffin-embedded human brain.Anti-S100A13 antibody was used at 1/1000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use. Counterstained with hematoxylin.               

      Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human testis.Anti-S100A13 antibody was used at 1/1000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use. Counterstained with hematoxylin.               

      Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human thyroid.Anti-S100A13 antibody was used at 1/1000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use. Counterstained with hematoxylin.       

      Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human tonsil.Anti-S100A13 antibody was used at 1/1000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use. Counterstained with hematoxylin.               

      Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human thyroid cancer.Anti-S100A13 antibody was used at 1/1000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use. Counterstained with hematoxylin.       

      Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human melanoma.Anti-S100A13 antibody was used at 1/1000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use. Counterstained with hematoxylin.       

      Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.