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CD9 Recombinant Rabbit mAb (SDT-143-53)

CD9 Recombinant Rabbit mAb (SDT-143-53)

货号: S0B0027
价格: 600
规格: 25μl
介绍: -
其他: -
产品规格

  • 宿主来源

    Rabbit

  • 抗原名称

    CD9

  • 免疫原

    Synthetic Peptide

  • 细胞定位

    Cell membrane

  • Accession

    P21926

  • 克隆号

    SDT-143-53

  • 抗体类型

    Rabbit mAb

  • 应用

    IHC-P, FCM, WB, IP

  • 反应种属 ?

    Hu

  • 纯化方式

    Protein A

  • 浓度

    0.5 mg/ml

  • 性状

    Liquid

  • 缓冲体系

    PBS, 40% Glycerol, 0.05%BSA, 0.03% Proclin 300

  • 储存条件

    12 months from date of receipt / reconstitution, -20 °C as supplied
稀释度
应用 稀释度
WB 1:1000
IHC-P 1:500-2000
FCM 1:500
IP 1:25
背景介绍

  • CD9 belongs to the cell surface glycoprotein cross -membrane four -protein family, with four cross -membrane domains, a shorter outer domain (ECL1), and a longer extracellular domain (ECL2). It is expressed in a variety of hematopoietic cells and epithelial cells. For example, Pre B cells, B cell subset, activated T cells, basophils, eosinophils, macrophages, megakaryocytes, plasma cells, plasma cell precursors in germinal centers, and platelets. Broadcasting a series of cell processes such as cell adhesion, movement, membrane tissue and signal transfusion. The lowering of CD9 expression is related to the adverse prognosis and progress of various cancers. It is a favorable marker of gallbladder cancer, gastic GIST, malignant cortex and oral squamous cell carcinoma.

  • 免疫印迹

    • WB result of CD9 Rabbit mAb                 

      Primary antibody: CD9 Rabbit mAb at 1/1000 dilution
      Lane 1: Raji whole cell lysate 20 µg
      Lane 2: K562 whole cell lysate 20 µg
      Lane 3: Hela whole cell lysate 20 µg
      Lane 4: HCT 116 whole cell lysate 20 µg
      Lane 5: MCF7 whole cell lysate 20 µg

      Negative control:

      Raji whole cell lysate;                                   

      K562 whole cell lysate                       

      Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 22 kDa
      Observed MW: 22 kDa

  • 流式分析

    • Flow cytometric analysis of  Raji (left) / HCT 116 (right) cells labelling CD9 antibody at 1/500 (0.1ug) dilution/ (red) compared with a Rabbit monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti-Rabbit IgG Alexa Fluor® 488 was used as the secondary antibody. Negative control: Raji

  • 免疫沉淀

    • CD9 Rabbit mAb at 1/25 dilution (2µg) immunoprecipitating CD9 in 0.4mg MCF7 whole cell lysate.
      Western blot was performed on the immunoprecipitate using CD9 Rabbit mAb at 1/1000 dilution.
      Secondary antibody (HRP) for IP was used at 1/400 dilution.
      Lane 1 : MCF7 whole cell lysate 10µg (input)
      Lane 2 : CD9 Rabbit mAb IP in MCF7 whole cell lysate
      Lane 3 : Rabbit monoclonal IgG IP in MCF7 whole cell lysate
      Predicted MW: 22 kDa
      Observed MW: 22 kDa

  • 免疫组化

    • IHC shows positive staining in paraffin-embedded human tonsil.Anti-CD9 antibody was used at 1/500 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use. Counterstained with hematoxylin.               

      Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human spleen.Anti-CD9 antibody was used at 1/500 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use. Counterstained with hematoxylin.               

      Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human prostate.Anti-CD9 antibody was used at 1/500 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use. Counterstained with hematoxylin.     

      Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human human ovarian cancer.Anti-CD9 antibody was used at 1/500 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use. Counterstained with hematoxylin.

      Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human human lung squamous cancer.Anti-CD9 antibody was used at 1/2000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use. Counterstained with hematoxylin.                                                 

      Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human breast cancer.Anti-CD9 antibody was used at 1/2000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use. Counterstained with hematoxylin.     

      Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human bladder cancer.Anti-CD9 antibody was used at 1/500 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use. Counterstained with hematoxylin.       

      Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • Negative control:IHC shows negative staining in paraffin-embedded human placenta.Red blood cells is negative.Anti-CD9 antibody was used at 1/500 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use. Counterstained with hematoxylin.                       

      Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.