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LIV-1/SLC39A6 Recombinant Rabbit mAb (SDT-206-84)

LIV-1/SLC39A6 Recombinant Rabbit mAb (SDT-206-84)

货号: S0B0023
价格: 260
规格: 10μl
介绍: -
其他: -
产品规格
  • 宿主来源

    Rabbit
  • 抗原名称

    LIV-1
  • 分子别名

    Zinc transporter ZIP6, Estrogen-regulated protein LIV-1, Solute carrier family 39 member 6, Zrt- and Irt-like protein 6, ZIP-6
  • 免疫原

    Synthetic Peptide
  • 细胞定位

    Cytoplasm, Membrane
  • Accession

    Q13433
  • 克隆号

    SDT-206-84
  • 抗体类型

    Rabbit mAb
  • 应用

    IHC-P, WB
  • 反应种属 ?

    Hu
  • 预测反应种属
    (反应种属缩写表)

    Or
  • 纯化方式

    Protein A
  • 浓度

    0.5 mg/ml
  • 标记

    Unconjugated
  • 性状

    Liquid
  • 缓冲体系

    PBS, 40% Glycerol, 0.05%BSA, 0.03% Proclin 300

  • 储存条件

    12 months from date of receipt / reconstitution, -20 °C as supplied

稀释度
应用 稀释度
WB 1:1000
IHC-P 1:200
背景介绍
  • Zinc transporter LIV-1 (SLC39A6) is estrogen regulated and present in increased amounts in estrogen receptor-positive breast cancer as well as in tumors that spread to the lymph nodes. The LIV-1 subfamily of ZIP zinc transporters consists of nine human sequences that share considerable homology across transmembrane domains. Many of these sequences have been shown to transport zinc and/or other ions across cell membranes. SLC39A6 is a member of the ZIP family of transporters, which control zinc homeostasis by regulating the influx of zinc from extracellular to intracellular spaces. The zinc transport function of SLC39A6 plays an important role in cellular metabolism. Zinc is required for a variety of cellular processes, including immune activity, protein synthesis, nucleic acid metabolism, cell proliferation, tissue repair and cell division, and low zinc levels can lead to metabolic disorder and inhibit cell growth. Overexpression of SLC39A6 has been related to the progression of several types of cancer, including breast , prostate, pancreatic, cervical and liver cancer, verexpression of SLC39A6 promoted the epithelial-mesenchymal transition (EMT).

  • 免疫印迹

    • WB result of LIV-1/SLC39A6 Rabbit mAb                

      Primary antibody: LIV-1/SLC39A6 Rabbit mAb at 1/1000 dilution
      Lane 1: MCF7 whole cell lysate 16 µg
      Lane 2: MCF7 whole cell lysate (deglycosylated with PNGase F) 16 μg

      Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 85 kDa
      Observed MW: 103/85/68 kDa
      Exposure time: 180s

  • 免疫组化

    • IHC shows positive staining in paraffin-embedded human breast cancer.

      Anti-LIV-1/SLC39A6 antibody was used at 1/200 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use. Counterstained with hematoxylin.

      Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human breast cancer.

      Anti-LIV-1/SLC39A6 antibody was used at 1/200 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use. Counterstained with hematoxylin.

      Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human breast. Anti-LIV-1/SLC39A6 antibody was used at 1/200 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use. Counterstained with hematoxylin.

      Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.