PBS, 40% Glycerol, 0.05%BSA, 0.03% Proclin 300
12 months from date of receipt / reconstitution, -20 °C as supplied
| 应用 | 稀释度 |
|---|---|
| WB | 1:5000-1:50000 |
| ICFCM | 1:500 |
| ICC | 1:500 |
HA-tag is derivative of self -influenza hemaginin, which is widely used as general table tags in the expression carrier.
WB result of HA tag Recombinant Rabbit mAb (SDT-113-76)
Lysate: GST Protein, HA tag 20 ng
Primary antibody:
Lane 1: HA tag Recombinant Rabbit mAb at 1/5000 dilution
Lane 2: HA tag Recombinant Rabbit mAb at 1/10000 dilution
Lane 3: HA tag Recombinant Rabbit mAb at 1/50000 dilution
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Flow cytometric analysis of 4% PFA fixed 0.1% Tween-20 permeabilized Histone H3, GST Tag, HA Tag, Myc tag transfected 293T (Human embryonic kidney epithelial cell) labelling HA tag antibody at 1/500 (0.1 μg) dilution/ (right panel) compared with a Rabbit IgG Isotype Control / (left panel). Goat Anti-Rabbit IgG Alexa Fluor® 488 was used as the secondary antibody. Then cells were stained with Myc - Alexa Fluor® 647 separately.
ICC shows positive staining in Histone H3-HA Tag transfected 293T cells (left panel) and negative staining in vector-transfected 293T cells (right panel). Anti-HA tag antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
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