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TCL1 Recombinant Rabbit mAb(SDT-052-12)

TCL1 Recombinant Rabbit mAb(SDT-052-12)

货号: S0B2045
价格: 600
规格: 25μl
介绍: -
其他: -
产品规格

  • 宿主来源

    Rabbit

  • 抗原名称

    TCL1

  • 分子别名

    Oncogene TCL-1, Protein p14 TCL1

  • 免疫原

    Synthetic Peptide

  • 细胞定位

    Cytoplasm, Nucleus

  • Accession

    P56279

  • 克隆号

    SDT-052-12

  • 抗体类型

    Rabbit mAb

  • 应用

    IHC-P, WB, IP

  • 反应种属 ?

    Hu

  • 纯化方式

    Protein A

  • 浓度

    0.5mg/ml

  • 标记

    Unconjugated

  • 性状

    Liquid

  • 缓冲体系

    PBS, 40% Glycerol, 0.05%BSA, 0.03% Proclin 300

  • 储存条件

    12 months from date of receipt / reconstitution, -20 °C as supplied
稀释度
应用 稀释度
IHC-P 1:2000
WB 1:1000
IP 1:25
背景介绍

  • T-cell leukemia/lymphoma protein 1A is a protein that in humans is encoded by the TCL1A gene. The TCL1 locus on human chromosome 14q32.1 is activated in T-cell leukemias by translocations and inversions that juxtapose it to regulatory elements of T-cell receptor genes. TCL1A has been shown to interact with AKT1 and AKT2. Protein expression in immature T and B lymphoid cells and overexpression of TCL1 is considered critical in the oncogenetic transformation.

  • 免疫印迹

    • WB result of TCL1 Rabbit mAb                

      Primary antibody: TCL1 Rabbit mAb at 1/1000 dilution
      Lane 1: HL-60 whole cell lysate 20 µg
      Lane 2: Raji whole cell lysate 20 µg
      Lane 3: Ramos whole cell lysate 20 µg
      Lane 4: Daudi whole cell lysate 20 µg
      Negative control: HL-60 whole cell lysate        

      Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 13 kDa
      Observed MW: 13 kDa
      Exposure time: 6s

  • 免疫沉淀

    • TCL1 Rabbit mAb at 1/25 dilution (2µg) immunoprecipitating TCL1 in 0.4mg Daudi whole cell lysate. Western blot was performed on the immunoprecipitate using TCL1 Rabbit mAb at 1/1000 dilution. Secondary antibody (HRP) for IP was used at 1/400 dilution.
      Lane 1: Daudi whole cell lysate 10µg (input)
      Lane 2: TCL1 Rabbit mAb IP in Daudi whole cell lysate
      Lane 3: Rabbit monoclonal IgG IP in Daudi whole cell lysate
      Predicted MW: 13 kDa
      Observed MW: 13 kDa
      Exposure time: 72s

  • 免疫组化

    • IHC shows positive staining in paraffin-embedded human tonsil.

      Anti-TCL1 antibody was used at 1/2000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use. Counterstained with hematoxylin.     

      Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human spleen.

      Anti-TCL1 antibody was used at 1/2000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use. Counterstained with hematoxylin.     

      Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.