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Histone H3 (Crotonyl K9) Recombinant Rabbit mAb (S-R409)

Histone H3 (Crotonyl K9) Recombinant Rabbit mAb (S-R409)

货号: S0B0879
价格: 600
规格: 25μl
介绍: -
其他: -
产品规格
  • 宿主来源

    Rabbit
  • 抗原名称

    Histone H3 (Crotonyl K9)
  • 分子别名

    H3K9Cr
  • 细胞定位

    Nucleus
  • Accession

    P68431
  • 克隆号

    S-R409
  • 抗体类型

    Recombinant mAb
  • 抗体同种型

    IgG
  • 应用

    ICC, WB, IP
  • 反应种属 ?

    Hu
  • 纯化方式

    Protein A
  • 浓度

    1 mg/ml
  • 标记

    Unconjugated
  • 性状

    Liquid
  • 缓冲体系

    PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
  • 储存条件

    12 months from date of receipt / reconstitution, -20 °C as supplied

稀释度
应用 稀释度
WB 1:500-1:1000
IP 1:100
ICC 1:1000
背景介绍
  • Histone H3 crotonylation, specifically at lysine 9 (H3K9cr), is a form of post-translational modification that has been identified as a key player in epigenetic regulation, particularly in plants 。This modification is structurally and functionally distinct from the well-studied lysine acetylation and is involved in various biological processes, including chromatin remodeling, metabolism, cell cycle, and cellular organization. In the context of histones, crotonylation can be found on different lysine residues among plant species and is known to overlap with the histone modification H3K9ac in about 95% of the regions, which suggests a potential interplay between these two modifications.

  • 免疫印迹

    • WB result of Histone H3 (Crotonyl K9) Recombinant Rabbit mAb
      Primary antibody: Histone H3 (Crotonyl K9) Recombinant Rabbit mAb at 1/1000 dilution
      Lane 1: untreated HeLa whole cell lysate 20 µg
      Lane 2: HeLa treated with 500 ng/ml TSA for 4 hours whole cell lysate 20 µg
      Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 15 kDa
      Observed MW: 17 kDa
      This blot was developed with high sensitivity substrate

  • 免疫沉淀

    • Histone H3 (Crotonyl K9) Rabbit mAb at 1/100 dilution (1 µg) immunoprecipitating Histone H3 (Crotonyl K9) in 0.4 mg HeLa treated with 500 ng/ml TSA for 4 hours whole cell lysate.
      Western blot was performed on the immunoprecipitate using Histone H3 (Crotonyl K9) Rabbit mAb at 1/1000 dilution.
      Secondary antibody (HRP) for IP was used at 1/1000 dilution.
      Lane 1: HeLa treated with 500 ng/ml TSA for 4 hours whole cell lysate 20 µg (Input)
      Lane 2: Histone H3 (Crotonyl K9) Rabbit mAb IP in HeLa treated with 500 ng/ml TSA for 4 hours whole cell lysate
      Lane 3: Rabbit monoclonal IgG IP in HeLa treated with 500 ng/ml TSA for 4 hours whole cell lysate
      Predicted MW: 15 kDa
      Observed MW: 17 kDa
      This blot was developed with high sensitivity substrate

  • 免疫细胞化学

    • ICC analysis of HeLa cells treated with TSA (500ng/ml,4h) (top panel) and HeLa cells untreated with TSA (500ng/ml,4h) (below panel). Anti-Histone H3 (Crotonyl K9) antibody was used at 1/1000 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).