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Galectin-1/LGALS1 Rabbit Polyclonal Antibody

Galectin-1/LGALS1 Rabbit Polyclonal Antibody

货号: S0B0844
价格: 600
规格: 25μl
介绍: -
其他: -
产品规格
  • 宿主来源

    Rabbit
  • 抗原名称

    Galectin-1/LGALS1
  • 分子别名

    Gal-1, 14 kDa laminin-binding protein (HLBP14), 14 kDa lectin, Beta-galactoside-binding lectin L-14-I, Galaptin, HBL, HPL, Lactose-binding lectin 1, Lectin galactoside-binding soluble 1, Putative MAPK-activating protein PM12, S-Lac lectin 1, LGALS1
  • 免疫原

    Synthetic Peptide
  • 细胞定位

    Cytoplasm, Secreted
  • Accession

    P09382
  • 抗体同种型

    IgG
  • 应用

    IHC-P, WB
  • 反应种属 ?

    Hu, Ms, Rt
  • 纯化方式

    Immunogen Affinity
  • 浓度

    4 mg/ml
  • 标记

    Unconjugated
  • 性状

    Liquid
  • 缓冲体系

    PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
  • 储存条件

    12 months from date of receipt / reconstitution, -20 °C as supplied

稀释度
应用 稀释度
WB 1:1000
IHC-P 1:1000
背景介绍
  • Galectin-1, a member of the galectin family of proteins, is a versatile and multifunctional molecule that plays pivotal roles in various biological processes. Galectin-1 supports tumor growth and metastasis by promoting tumor angiogenesis, inhibiting T-cell-mediated cytotoxic immune responses, and other mechanisms. The expression or overexpression of Galectin-1 in tumor and/or tumor-adjacent tissues serves as a marker of malignant progression and a potential diagnostic, prognostic, and therapeutic biomarker for cancer. Galectin-1 exhibits pleiotropic binding activities, modulating diverse immune cells primarily to promote immune tolerance and downregulate innate and adaptive immune responses. Galectin-1 is upregulated during inflammation and infection, modulating immune cells under both physiological and pathological conditions. It controls immune cell homeostasis and regulates acute and chronic inflammation by inhibiting the synthesis of proinflammatory cytokines, participating in T cell apoptosis programs, promoting the expansion of regulatory T cells (Tregs), and inactivating antigen-presenting cells. Galectin-1 is also essential for a healthy pregnancy and serves as a valuable biomarker for early diagnosis of preeclampsia (PE).

  • 免疫印迹

    • WB result of Galectin-1 Rabbit pAb
      Primary antibody: Galectin-1 Rabbit pAb at 1/1000 dilution
      Lane 1: LNCaP whole cell lysate 20 µg
      Lane 2: HeLa whole cell lysate 20 µg
      Lane 3: A549 whole cell lysate 20 µg
      Negative control: LNCaP whole cell lysate
      Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 15 kDa
      Observed MW: 13 kDa

    • WB result of Galectin-1 Rabbit pAb
      Primary antibody: Galectin-1 Rabbit pAb at 1/1000 dilution
      Lane 1: NIH/3T3 whole cell lysate 20 µg
      Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 15 kDa
      Observed MW: 13 kDa

    • WB result of Galectin-1 Rabbit pAb
      Primary antibody: Galectin-1 Rabbit pAb at 1/1000 dilution
      Lane 1: PC-12 whole cell lysate 20 µg
      Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 15 kDa
      Observed MW: 13 kDa

  • 免疫组化

    • IHC shows positive staining in paraffin-embedded human kidney. Anti-Galectin-1/LGALS1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human tonsil. Anti-Galectin-1/LGALS1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human breast cancer. Anti-Galectin-1/LGALS1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human colon cancer. Anti-Galectin-1/LGALS1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.