The lyophilized product remains stable up to 1 year at -20 °C from date of receipt. Upon reconstitution, it can be stored for 2-3 weeks at 2-8 °C or for up to 12 months at -20 °C or below. Avoid repeated freezing and thawing cycles.
| 应用 | 稀释度 |
|---|---|
| ELISA | 0.005-0.05 µg/ml |
Pembrolizumab, sold under the brand name Keytruda, is a humanized antibody used in cancer immunotherapy that treats melanoma, lung cancer, head and neck cancer, Hodgkin lymphoma, stomach cancer, cervical cancer, and certain types of breast cancer. It is administered by slow intravenous injection. It is an IgG4 isotype antibody that blocks a protective mechanism of cancer cells, allowing the immune system to destroy them. It targets the programmed cell death protein 1 (PD-1) receptor of lymphocytes by blocking its interaction with PD-L1, resulting in the activation of T-cell-mediated immune responses against tumor cells.
ELISA binding of Anti-Pembrolizumab Recombinant Rabbit mAb (SDT-825-21) with Pembrolizumab. While the antibody does not recognize the human IgG4 control and human total IgG (data not shown).
Coating antigen: Pembrolizumab, 0.2 µg/ml.
Anti-Pembrolizumab Recombinant Rabbit mAb (SDT-825-21) (S0B0840) dilutions start from 500 ng/ml.
EC50= 4.229 ng/ml.
Anti-Pembrolizumab Recombinant Rabbit mAb (SDT-825-21) (S0B0840) blocks Pembrolizumab binding with PD-1 Fc Chimera Protein, Human (UA010002, STARTER).
Coating antigen: Pembrolizumab, 1 µg/ml
PD-1-Fc-biotin final concentration: 5 ng/ml
Anti-Pembrolizumab Recombinant Rabbit mAb (SDT-825-21) (S0B0840) dilutions start from 40 μg/ml.
IC50= 343.4 ng/ml.
Standard curve of Pembrolizumab Sandwich ELISA. The Pembrolizumab Sandwich ELISA assay is developed by using Anti-Pembrolizumab Recombinant Rabbit mAb (SDT-825-61) (S0B0842) and Anti-Pembrolizumab Recombinant Rabbit mAb (SDT-825-21) (S0B0840) as the capture and detection antibodies, respectively.
In this ELISA assay, Anti-Pembrolizumab Recombinant Rabbit mAb (SDT-825-21) (S0B0840) was labeled with Biotin.
The sensitivity of detecting Pembrolizumab is up to 23 pg/ml.
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