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p38 gamma/MAPK12 Recombinant Rabbit mAb (S-1253-50)

p38 gamma/MAPK12 Recombinant Rabbit mAb (S-1253-50)

货号: S0B0801
价格: 260
规格: 10μl
介绍: -
其他: -
产品规格

  • 宿主来源

    Rabbit

  • 抗原名称

    p38 gamma/MAPK12

  • 分子别名

    Mitogen-activated protein kinase 12; MAP kinase 12; Extracellular signal-regulated kinase 6 (ERK-6); Mitogen-activated protein kinase p38 gamma (MAP kinase p38 gamma); Stress-activated protein kinase 3; ERK6; SAPK3

  • 免疫原

    Synthetic Peptide

  • 细胞定位

    Cytoplasm, Nucleus

  • Accession

    P53778

  • 克隆号

    S-1253-50

  • 抗体类型

    Recombinant mAb

  • 抗体同种型

    IgG

  • 应用

    ICFCM, ICC, WB

  • 反应种属 ?

    Hu

  • 纯化方式

    Protein A

  • 浓度

    0.5 mg/ml

  • 标记

    Unconjugated

  • 性状

    Liquid

  • 缓冲体系

    PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300

  • 储存条件

    12 months from date of receipt / reconstitution, -20 °C as supplied

稀释度
应用 稀释度
WB 1:1000
ICC 1:500
ICFCM 1:50
背景介绍

  • MAPK12, also known as ERK3, ERK6, P38GAMMA, PRKM12, SAPK-3, or SAPK3, belongs to the essential family of Mitogen-Activated Protein Kinases (MAPKs). is involved in intracellular signaling cascades that respond to various stimuli. Upon activation, it phosphorylates downstream targets, modulating their activity and ultimately affecting cellular processes.

  • 免疫印迹

    • WB result of p38 gamma / MAPK12 Recombinant Rabbit mAb
      Primary antibody: p38 gamma / MAPK12 Recombinant Rabbit mAb at 1/1000 dilution
      Lane 1: 293T whole cell lysate 20 µg
      Lane 2: HeLa whole cell lysate 20 µg
      Lane 3: Jurkat whole cell lysate 20 µg
      Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 41 kDa
      Observed MW: 45 kDa

    • WB result of p38 gamma / MAPK12 Recombinant Rabbit mAb
      Primary antibody: p38 gamma / MAPK12 Recombinant Rabbit mAb at 1/1000 dilution
      Lane 1: PC-12 whole cell lysate 20 µg
      Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 41 kDa
      Observed MW: 40 kDa

  • 流式分析

    • Flow cytometric analysis of 4% PFA fixed 90% methanol permeabilized HeLa (Human cervix adenocarcinoma epithelial cell) labelling p38 gamma / MAPK12 antibody at 1/50 dilution (1 μg)/ (Red) compared with a Rabbit monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti - Rabbit IgG Alexa Fluor® 488 was used as the secondary antibody.

  • 免疫细胞化学

    • ICC shows positive staining in HeLa cells. Anti- p38 gamma/MAPK12 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).