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PKC eta Recombinant Rabbit mAb (S-1053-35)

PKC eta Recombinant Rabbit mAb (S-1053-35)

货号: S0B0790
价格: 260
规格: 10μl
介绍: -
其他: -
产品规格

  • 宿主来源

    Rabbit

  • 抗原名称

    PKC eta

  • 分子别名

    Protein kinase C eta type, PKC-L, nPKC-eta, Prkch, Pkch

  • 免疫原

    Synthetic Peptide

  • 细胞定位

    Cytoplasm

  • Accession

    P23298

  • 克隆号

    S-1053-35

  • 抗体类型

    Recombinant mAb

  • 抗体同种型

    IgG

  • 应用

    WB, IP

  • 反应种属 ?

    Hu, Ms, Rt

  • 纯化方式

    Protein A

  • 浓度

    0.5 mg/ml

  • 标记

    Unconjugated

  • 性状

    Liquid

  • 缓冲体系

    PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300

  • 储存条件

    12 months from date of receipt / reconstitution, -20 °C as supplied

稀释度
应用 稀释度
WB 1:1000
IP 1:50
背景介绍

  • PKC eta, also known as Protein Kinase C eta, is a member of the Protein Kinase C (PKC) family of enzymes. This serine/threonine-specific protein kinase plays crucial roles in various cellular processes, particularly in signal transduction, cell differentiation, and gene transcription regulation. PKC eta regulates keratinocyte differentiation by activating the MAPK13 (p38delta) activated protein kinase cascade. PKC eta mediates transcriptional activation of the transglutaminase 1 (TGM1) gene. Mutations in the gene have been linked to susceptibility to cerebral infarction.

  • 免疫印迹

    • WB result of PKC eta Recombinant Rabbit mAb
      Primary antibody: PKC eta Recombinant Rabbit mAb at 1/1000 dilution
      Lane 1: MCF7 whole cell lysate 20 µg
      Lane 2: PC-3 whole cell lysate 20 µg
      Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 78 kDa
      Observed MW: 45, 78 kDa
      This blot was developed with high sensitivity substrate

    • WB result of PKC eta Recombinant Rabbit mAb
      Primary antibody: PKC eta Recombinant Rabbit mAb at 1/1000 dilution
      Lane 1: mouse lung lysate 20 µg
      Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 78 kDa
      Observed MW: 45, 78 kDa
      This blot was developed with high sensitivity substrate

    • WB result of PKC eta Recombinant Rabbit mAb
      Primary antibody: PKC eta Recombinant Rabbit mAb at 1/1000 dilution
      Lane 1: rat lung lysate 20 µg
      Lane 2: rat skin lysate 20 µg
      Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 78 kDa
      Observed MW: 45, 78 kDa
      This blot was developed with high sensitivity substrate

  • 免疫沉淀

    • PKC eta Rabbit mAb at 1/50 dilution (1 µg) immunoprecipitating PKC eta in 0.4 mg mouse lung lysate.
      Western blot was performed on the immunoprecipitate using PKC eta Rabbit mAb at 1/1000 dilution.
      Secondary antibody (HRP) for IP was used at 1/1000 dilution.
      Lane 1: mouse lung lysate 20 µg (Input)
      Lane 2: PKC eta Rabbit mAb IP in mouse lung lysate
      Lane 3: Rabbit monoclonal IgG IP in mouse lung lysate
      Predicted MW: 78 kDa 
      Observed MW: 45, 78 kDa