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Invivo mouse IgG1 isotype control (D265A)

Invivo mouse IgG1 isotype control (D265A)

货号: S0B0788
价格: 700
规格: 1mg
介绍: -
其他: -
产品规格

  • 宿主来源

    Mouse

  • 克隆号

    SDT-844-79H(D265A)L

  • 抗体类型

    Mouse mAb

  • 突变

    D265A

  • 应用

    In vivo control, IHC-P, FC, WB

  • 纯化方式

    Protein G

  • 浓度

    Lot specific* (generally 5 to 20 mg/ml)*

  • 内毒素含量

    <2EU/mg

  • 标记

    Unconjugated

  • 性状

    Liquid

  • 缓冲体系

    PBS pH7.4, containing no preservative

  • 储存条件

    2 to 8 °C for 2 weeks under sterile conditions;

    -20 °C for 3 months under sterile conditions; 

    -80 °C for 24 months under sterile conditions.

    Please avoid repeated freeze-thaw cycles.

稀释度
应用 稀释度
WB 1:1000
ICFCM 1:200
FCM 1:200
背景介绍

  • Isotype control antibodies, to estimate the nonspecific binding of target.

  • 免疫印迹

    • WB result of Invivo mouse IgG1 isotype control (D265A)
      Primary antibody: Invivo mouse IgG1 isotype control (D265A) at 1/1000 dilution
      Lane 1: HeLa whole cell lysate 20 µg
      Lane 2: THP-1 whole cell lysate 20 µg
      Secondary antibody: Goat Anti-mouse IgG, (H+L), HRP conjugated at 1/10000 dilution

    • WB result of Invivo mouse IgG1 isotype control (D265A)
      Primary antibody: Invivo mouse IgG1 isotype control (D265A) at 1/1000 dilution
      Lane 1: mouse brain lysate 20 µg
      Secondary antibody: Goat Anti-mouse IgG, (H+L), HRP conjugated at 1/10000 dilution

    • WB result of Invivo mouse IgG1 isotype control (D265A)
      Primary antibody: Invivo mouse IgG1 isotype control (D265A) at 1/1000 dilution
      Lane 1: rat brain lysate 20 µg
      Secondary antibody: Goat Anti-mouse IgG, (H+L), HRP conjugated at 1/10000 dilution

  • 流式分析

    • Flow cytometric analysis of THP-1 (Human monocytic leukemia monocyte) labeling mouse IgG1 isotype control (D265A) at 1/200 (1 μg) dilution (Left) / (Red) compared with CD13 antibody at 1/200 (1 μg) dilution (S0B0441) (Right) / (Red), Mouse monoclonal IgG Isotype Control (Left) / (Black) compared with mouse IgG1 isotype control (Right) / (Black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti - Mouse IgG Alexa Fluor® 488 was used as the secondary antibody.

    • Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized NIH/3T3 (Mouse embryonic fibroblast) labelling mouse IgG1 isotype control (D265A) at 1/200 (1 μg) dilution (Left) / (Red) compared with α-tubulin antibody (Right) / (Red), Mouse monoclonal IgG Isotype Control (Left) / (Black) compared with mouse IgG1 isotype control (Right) / (Black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti - Mouse IgG Alexa Fluor® 488 was used as the secondary antibody.

  • 免疫组化

    • IHC shows negative staining in paraffin-embedded human kidney. Invivo mouse IgG1 isotype control (D265A) was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows negative staining in paraffin-embedded human liver. Invivo mouse IgG1 isotype control (D265A) was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows negative staining in paraffin-embedded mouse spleen. Invivo mouse IgG1 isotype control (D265A) was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.