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Phospho-p70 S6 Kinase (Ser371) Recombinant Rabbit mAb (S-1239-6)

Phospho-p70 S6 Kinase (Ser371) Recombinant Rabbit mAb (S-1239-6)

货号: S0B0784
价格: 600
规格: 25μl
介绍: -
其他: -
产品规格

  • 宿主来源

    Rabbit

  • 抗原名称

    Phospho-p70 S6 Kinase (Ser371)

  • 分子别名

    Ribosomal protein S6 kinase beta-1, S6K-beta-1, S6K1, 70 kDa ribosomal protein S6 kinase 1 (P70S6K1; p70-S6K 1), Ribosomal protein S6 kinase I, Serine/threonine-protein kinase 14A, p70 ribosomal S6 kinase alpha (p70 S6 kinase alpha; p70 S6K-alpha; p70 S6KA), RPS6KB1, STK14A

  • 免疫原

    Synthetic Peptide

  • 细胞定位

    Cytoplasm

  • Accession

    P23443

  • 克隆号

    S-1239-6

  • 抗体类型

    Recombinant mAb

  • 抗体同种型

    IgG

  • 应用

    WB, IP

  • 反应种属 ?

    Hu

  • 预测反应种属
    (反应种属缩写表)

    Bv, Rb, Rt, Ms

  • 纯化方式

    Protein A

  • 浓度

    0.5 mg/ml

  • 标记

    Unconjugated

  • 性状

    Liquid

  • 缓冲体系

    PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300

  • 储存条件

    12 months from date of receipt / reconstitution, -20 °C as supplied

稀释度
应用 稀释度
Dot Blot 1:1000
WB 1:1000
IP 1:50

  • 免疫印迹

    • WB result of Phospho-p70 S6 Kinase (Ser371) Recombinant Rabbit mAb
      Primary antibody: Phospho-p70 S6 Kinase (Ser371) Recombinant mAb at 1/1000 dilution
      Lane 1: 293T whole cell lysate 20 µg
      Lane 2: phosphatase treated 293T whole cell lysate 20 µg
      Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 59 kDa
      Observed MW: 55~70 kDa

  • 免疫沉淀

    • Phospho-p70 S6 Kinase (Ser371) Rabbit mAb at 1/50 dilution (1 µg) immunoprecipitating Phospho-p70 S6 Kinase (Ser371) in 0.4 mg 293T whole cell lysate.
      Western blot was performed on the immunoprecipitate using Phospho-p70 S6 Kinase (Ser371) Rabbit mAb at 1/1000 dilution.
      Secondary antibody (HRP) for IP was used at 1/1000 dilution.
      Lane 1: 293T whole cell lysate 20 µg (Input)
      Lane 2: Phospho-p70 S6 Kinase (Ser371) Rabbit mAb IP in 293T whole cell lysate
      Lane 3: Rabbit monoclonal IgG IP in 293T whole cell lysate
      Predicted MW: 59 kDa 
      Observed MW: 55~70 kDa

  • 斑点杂交

    • Dot blot result of Phospho-p70 S6 Kinase (Ser371) Recombinant mAb
      Lane 1: Phospho-p70 S6 Kinase (Ser371) peptide
      Lane 2: Phospho-p70 S6 Kinase unmodified peptide
      Primary antibody: Phospho-p70 S6 Kinase (Ser371) Recombinant mAb at 1/1000 dilution
      Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution