12 months from date of receipt / reconstitution, -20 °C as supplied
| 应用 | 稀释度 |
|---|---|
| WB | 1:5000-1:10000 |
Beta-actin (human gene and protein abbreviation ACTB/ACTB) is one of six different actin isoforms which have been identified in humans. This is one of the two nonmuscle cytoskeletal actins. Actins are highly conserved proteins that are involved in cell motility, structure and integrity. Beta actin is often used in Western blotting as a loading control, to normalize total protein amounts and check for eventual protein degradation in the samples. Its transcript is also commonly used as a housekeeping gene standard in qPCR. Its molecular weight is approximately 42 kDa.
WB result of β-actin Recombinant Rabbit mAb (HRP Conjugate)
Primary antibody: β-actin Recombinant Rabbit mAb (HRP Conjugate) at 1/10000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: HepG2 whole cell lysate 20 µg
Predicted MW: 42 kDa
Observed MW: 42 kDa
WB result of β-actin Recombinant Rabbit mAb (HRP Conjugate)
Primary antibody: β-actin Recombinant Rabbit mAb (HRP Conjugate) at 1/10000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Predicted MW: 42 kDa
Observed MW: 42 kDa
WB result of β-actin Recombinant Rabbit mAb (HRP Conjugate)
Primary antibody: β-actin Recombinant Rabbit mAb (HRP Conjugate) at 1/10000 dilution
Lane 1: C6 whole cell lysate 20 µg
Predicted MW: 42 kDa
Observed MW: 42 kDa
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