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Phospho-Akt (Ser473) Antibody Duo

Phospho-Akt (Ser473) Antibody Duo

货号: S0C2020
价格: 3780
规格: 1 kit (100μl/tube)
介绍: -
其他: -
产品规格

  • 宿主来源

    Rabbit

  • Accession

    P31751, Q9Y243, P31749

  • 抗体类型

    Recombinant mAb

  • 抗体同种型

    IgG

  • 应用

    WB, IP

  • 反应种属 ?

    Hu, Ms, Rt

  • 纯化方式

    Protein A

  • 浓度

    0.5 mg/ml

  • 标记

    Unconjugated

  • 性状

    Liquid

  • 缓冲体系

    PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300

  • 储存条件

    12 months from date of receipt / reconstitution, -20 °C as supplied

稀释度
应用 稀释度
WB 1:1000
IP 1:50
背景介绍

  • AKT protein, also known as Akt protein kinase B (PKB), is a serine/threonine-specific protein kinase that plays an important role in various cellular processes such as glucose metabolism, apoptosis, cell proliferation, transcription, and cell migration. AKT is particularly significant in cancer research as its overactivation can promote the survival and proliferation of various cancer cells. AKT participates in the PI3K/Akt signaling pathway and regulates various cellular functions such as metabolism, growth, proliferation, survival, transcription, and protein synthesis when fully activated. AKT can be activated by various upstream signals, including integrin, receptor tyrosine kinases (RTKs), B and T cell receptors, and G protein-coupled receptors. Conversely, protein phosphatase 2A (PP2A) can dephosphorylate and deactivate AKT.

  • 组分和操作说明

  • 免疫印迹

    • WB result of Akt (pan) Rabbit mAb
      Primary antibody: Akt (pan) Rabbit mAb at 1/1000 dilution
      Lane 1: GST-Akt1 protein 10 ng
      Lane 2: GST-Akt2 protein 10 ng
      Lane 3: GST-Akt3 protein 10 ng
      Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 72 kDa
      Observed MW: 60~100 kDa

    • WB result of Akt (pan) Rabbit mAb
      Primary antibody: Akt (pan) Rabbit mAb at 1/1000 dilution
      Lane 1: Jurkat whole cell lysate 20 µg
      Lane 2: MCF7 whole cell lysate 20 µg
      Lane 3: HeLa whole cell lysate 20 µg
      Lane 4: A549 whole cell lysate 20 µg
      Lane 5: NIH/3T3 whole cell lysate 20 µg
      Lane 6: C2C12 whole cell lysate 20 µg
      Lane 7: C6 whole cell lysate 20 µg
      Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 56 kDa
      Observed MW: 60 kDa

  • 免疫沉淀

    • Phospho-Akt (Ser473) Rabbit mAb at 1/50 dilution (1 µg) immunoprecipitating Phospho-Akt (Ser473) in 0.4 mg NIH/3T3 treated with calyculin A (100 nM, 30 min) whole cell lysate.
      Western blot was performed on the immunoprecipitate using Phospho-Akt (Ser473) Rabbit mAb at 1/1000 dilution.
      Secondary antibody (HRP) for IP was used at 1/400 dilution.
      Lane 1: NIH/3T3 treated with calyculin A (100 nM, 30 min) whole cell lysate 20 µg (Input)
      Lane 2: Phospho-Akt (Ser473) Rabbit mAb IP in NIH/3T3 treated with calyculin A (100 nM, 30 min) whole cell lysate
      Lane 3: Rabbit monoclonal IgG IP in NIH/3T3 treated with calyculin A (100 nM, 30 min) whole cell lysate
      Predicted MW: 56 kDa
      Observed MW: 60 kDa