12 months from date of receipt / reconstitution, -20 °C as supplied
| 应用 | 稀释度 |
|---|---|
| ICC | 1:100 |
| FCM | 1:50 |
CD24 is a small cell surface glycoprotein that belongs to the mucin-like family of proteins. It is expressed on various cell types, including hematopoietic stem cells, epithelial cells, endothelial cells, and a subpopulation of lymphocytes and monocytes. CD24 is involved in cell-cell interactions and cell adhesion. It can interact with other cell surface molecules, such as P-selectin, and play a role in cell migration and adhesion. CD24 expression is also associated with cancer progression and metastasis. It is overexpressed in many tumor types, including breast cancer, ovarian cancer, and colorectal cancer, and is often associated with poor prognosis. CD24 has been shown to regulate cell proliferation, apoptosis, and differentiation. It can activate signaling pathways, such as the PI3K/Akt pathway, and influence cellular responses to growth factors and cytokines.
Flow cytometric analysis of human PBMC (human peripheral blood mononuclear cell) labelling CD24 antibody at 1/50 (1 μg) dilution (Right) compared with a Rabbit monoclonal IgG isotype control (Left). Goat Anti - Rabbit IgG Alexa Fluor® 488 was used as the secondary antibody. Then cells were stained with CD3 - Alexa Fluor® 647 separately. Gated on total viable cells.
ICC shows positive staining in MCF-7 cells (top panel) and negative staining in MDA-MB-231 cells (below panel). Anti-CD24 antibody was used at 1/100 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
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