您现在的位置: 首页   -  产品中心   -  免疫学   -   抗体
CD200 Recombinant Rabbit mAb (S-1008-60)

CD200 Recombinant Rabbit mAb (S-1008-60)

货号: S0B0726
价格: 600
规格: 25μl
介绍: -
其他: -
产品规格
  • 宿主来源

    Rabbit
  • 抗原名称

    CD200
  • 分子别名

    OX-2 membrane glycoprotein, MOX1, MOX2
  • 免疫原

    Recombinant Protein
  • 细胞定位

    Cell membrane
  • Accession

    P41217
  • 克隆号

    S-1008-60
  • 抗体类型

    Recombinant mAb
  • 抗体同种型

    IgG
  • 应用

    IHC-P, FCM
  • 反应种属 ?

    Hu
  • 纯化方式

    Protein A
  • 浓度

    0.5 mg/ml
  • 标记

    Unconjugated
  • 性状

    Liquid
  • 缓冲体系

    PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
  • 储存条件

    12 months from date of receipt / reconstitution, -20 °C as supplied

稀释度
应用 稀释度
IHC-P 1:100
FCM 1:500
背景介绍
  • CD200 is a transmembrane glycoprotein belonging to the immunoglobulin superfamily that plays crucial roles in various biological processes. CD200 is expressed on a wide range of cell types, including B cells, certain subsets of T cells (including activated T cells), thymocytes, endothelial cells, and neurons. In normal B cells, CD200 is moderately expressed on peripheral blood B cells, bone marrow B-cell precursors, and reactive lymph node CD10-negative follicular extrafollicular center (EFC) B cells. It is weakly expressed on reactive lymph node CD10-positive follicular center (FC) B cells. In B-cell malignancies, the expression of CD200 is heterogeneous. For instance, it is highly expressed in hairy cell leukemia (HCL) and moderately to highly expressed in chronic lymphocytic leukemia (CLL) and Waldenström macroglobulinemia/lymphoplasmacytic lymphoma (LPL). However, the expression in large B-cell lymphomas (LBCL), follicular lymphomas (FL), and marginal zone lymphomas (MZL) varies significantly. CD200 produces immunosuppressive signals through binding to its cognate receptor, CD200 receptor 1 (CD200R1), which is expressed on granulocytes, monocytes, and subsets of T cells. This interaction plays a significant role in immune regulation. Studies have shown that CD200, acting through its receptor CD200R1, can inhibit excessive microglial activation induced by chronic stress, alleviate damage to adult hippocampal neurogenesis, and improve depressive-like behavior in mice, revealing a new mechanism of neuron-glial cell interaction in the occurrence of depression.

  • 流式分析

    • Flow cytometric analysis of DU145 (Human prostate carcinoma epithelial cell, left) / SK-MEL-28 (Human malignant melanoma, Right) labelling CD200 antibody at 1/500 dilution (0.1 μg) / (Red) compared with a Rabbit monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti - Rabbit IgG Alexa Fluor® 488 was used as the secondary antibody.
      Negative control: DU145

  • 免疫组化

    • IHC shows positive staining in paraffin-embedded human placenta. Anti-CD200 antibody was used at 1/100 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human tonsil. Anti-CD200 antibody was used at 1/100 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.