12 months from date of receipt / reconstitution, -20 °C as supplied
| 应用 | 稀释度 |
|---|---|
| WB | 1:1000 |
| ICC | 1:100 |
| ICFCM | 1:500 |
TRIM24 protein, also known as transcriptional intermediary factor 1 alpha (TIF1α), is a multifunctional co-activator protein that interacts dynamically with nuclear receptors and co-activators to influence target gene transcription. This protein belongs to the TRIM family of proteins, which are characterized by a conserved domain structure that includes RING, B-box, and coiled-coil regions. TRIM24 protein interacts with chromatin by recognizing specific histone H3 modifications, showing a high affinity for unmodified lysine 4 (H3K4me0) and acetylated lysine 23 (H3K23ac) of histone H3. This interaction is mediated by its bromodomain, which functions as a "reader" of epigenetic histone marks and regulates chromatin structure and gene expression by connecting relevant proteins to recognized acetylated histone targets. TRIM24 protein is an E3 ubiquitin protein ligase that promotes the proteasomal degradation of p53/TP53 and mediates cell proliferation and apoptosis along with TRIM33. It also regulates the transcriptional activation of retinoic acid (RA) receptors, including RARA, and has been shown to modulate RA-dependent hepatocyte proliferation. In addition, TRIM24 is the first transcription regulatory factor found to have a receptor target located in the nucleus. It can affect the expression and function of proteins related to chromatin remodeling by regulating them. TRIM24 can also utilize retinoic acid (RA) transcription to control tumorigenesis.
WB result of TRIM24 Rabbit mAb
Primary antibody: TRIM24 Rabbit mAb at 1/1000 dilution
Lane 1: HCT 116 whole cell lysate 20 µg
Lane 2: HeLa whole cell lysate 20 µg
Lane 3: COLO 205 whole cell lysate 20 µg
Lane 4: IMR-32 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 116 kDa
Observed MW: 140 kDa This blot was developed with high sensitivity substrate.
Flow cytometric analysis of 4% PFA fixed 90% methanol permeabilized HeLa (Human cervix adenocarcinoma epithelial cell) labelling TRIM24 antibody at 1/500 dilution (0.1 μg)/ (Red) compared with a Rabbit monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti - Rabbit IgG Alexa Fluor® 488 was used as the secondary antibody.
ICC shows positive staining in HeLa cells. Anti-TRIM24 antibody was used at 1/100 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
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