12 months from date of receipt / reconstitution, -20 °C as supplied
| 应用 | 稀释度 |
|---|---|
| WB | 1:5000-1:50000 |
| ICC | 1:50 |
The Glu-Glu tag is a labeling or marker sequence specific to the Glu-Glu dipeptide, consisting of two consecutive glutamic acid residues. This tag is commonly utilized in biological research and experiments for the purposes of tagging and identifying proteins or antibodies, allowing for their tracking and analysis within complex biological samples.
WB result of Glu-Glu tag Recombinant Rabbit mAb (S-905-4)
Lysate: Glu-Glu tagged fusion protein 20 ng
Primary antibody:
Lane 1: Glu-Glu tag Recombinant Rabbit mAb at 1/5000 dilution
Lane 2: Glu-Glu tag Recombinant Rabbit mAb at 1/10000 dilution
Lane 3: Glu-Glu tag Recombinant Rabbit mAb at 1/50000 dilution
Lane 4: Glu-Glu tag Recombinant Rabbit mAb at 1/100000 dilution
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
ICC shows positive staining in Histone H3-Glu-Glu Tag transfected 293T cells (top panel) and negative staining in vector-transfected 293T cells (below panel). Anti- Glu-Glu tag antibody was used at 1/50 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
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