12 months from date of receipt / reconstitution, -20 °C as supplied
| 应用 | 稀释度 |
|---|---|
| WB | 1:1000 |
| IHC-P | 1:500 |
CD272, also known as B and T lymphocyte attenuator (BTLA), is an antigen closely related to immune cell function. When the body is stimulated by antigenic substances, B-cells differentiate into plasma cells, producing immunoglobulins that can specifically bind to the corresponding antigens. CD272, as the B and T lymphocyte attenuator antigen, may play an important role in this process. In recent years, the application of CD272 in medical research has gradually increased. For example, in studies of acute myeloid leukemia, scientists have discovered that leukemia stem cells highly express CD272, and its high expression is negatively correlated with poor prognosis and survival rates of patients. This discovery provides a new target and research idea for the treatment of leukemia. By blocking the cross-linking signal between CD272 and its ligand, the growth of leukemia cells can be effectively inhibited, providing new possibilities for the treatment of leukemia.
WB result of BTLA/CD272 Rabbit mAb
Primary antibody: BTLA/CD272 Rabbit mAb at 1/1000 dilution
Lane 1: A20 whole cell lysate 20 µg
Lane 2: mouse spleen lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 34 kDa
Observed MW: 45~70 kDa
WB result of BTLA/CD272 Rabbit mAb
Primary antibody: BTLA/CD272 Rabbit mAb at 1/1000 dilution
Lane 1: rat spleen lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 34 kDa
Observed MW: 55~70 kDa
IHC shows positive staining in paraffin-embedded mouse spleen. Anti- BTLA/CD272 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
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