PBS pH7.4, containing no preservative
2 to 8 °C for 2 weeks under sterile conditions;
-20 °C for 3 months under sterile conditions;
-80 °C for 24 months under sterile conditions.
Please avoid repeated freeze-thaw cycles.
| 应用 | 稀释度 |
|---|---|
| WB | 1:50 |
| FCM | 1:50000 |
CD8a, also known as CD8α, is a molecular marker on the surface of T lymphocytes. It belongs to the family of leukocyte differentiation antigens and is primarily expressed on the surface of cytotoxic T lymphocytes (CTLs). CD8a plays a crucial role in immune responses related to cytotoxicity and local inflammation. It has the ability to bind to MHC class I molecules. The expression level of CD8 molecules undergoes changes during various stages of T cell differentiation, development, and in response to T cell stimuli. Additionally, CD8a is closely associated with autoimmune monitoring, humoral immune responses, and transplant reactions. For instance, CD8a can serve as a monitoring indicator for certain autoimmune diseases and plays a significant role in immunotherapy for anti-tumor and anti-viral treatments.
WB result of Invivo anti-mouse CD8α Rat mAb
Primary antibody: Invivo anti-mouse CD8α Rat mAb at 1/50 dilution
Lane 1: Mouse CD8, His tag (S0A1018) 1 µg
Lane 2: Mouse CD8, His tag (S0A1018) 0.5 µg
Secondary antibody: Goat Anti-rat IgG, (H+L), HRP conjugated at 1/1000 dilution
Predicted MW: 35 kDa
Observed MW: 25~35 kDa
Flow cytometric analysis of C57BL/6 mouse splenocytes labeling CD8α at 1/50000 (0.01 μg) dilution / (Right panel) compared with a Rat IgG, Isotype Control / (Left panel). Goat Anti - Rat IgG Alexa Fluor® 488 was used as the secondary antibody. Then cells were stained with CD3 - Alexa Fluor® 647 separately. Gated on total viable cells.
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