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p90RSK Rabbit polyclonal antibody

p90RSK Rabbit polyclonal antibody

货号: S0B0649
价格: 600
规格: 25μl
介绍: -
其他: -
产品规格
  • 宿主来源

    Rabbit
  • 分子别名

    Ribosomal protein S6 kinase alpha-1, S6K-alpha-1, 90 kDa ribosomal protein S6 kinase 1 (p90-RSK 1; p90RSK1; p90S6K), MAP kinase-activated protein kinase 1a (MAPK-activated protein kinase 1a; MAPKAP kinase 1a; MAPKAPK-1a), Ribosomal S6 kinase 1 (RSK-1), RPS6KA1, MAPKAPK1A, RSK1
  • 免疫原

    Synthetic Peptide
  • 细胞定位

    Cytoplasm, Nucleus
  • Accession

    Q15418
  • 抗体同种型

    IgG
  • 应用

    IHC-P, WB, IP
  • 反应种属 ?

    Hu, Ms, Rt
  • 纯化方式

    Protein A
  • 浓度

    0.5 mg/ml
  • 标记

    Unconjugated
  • 性状

    Liquid
  • 缓冲体系

    PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
  • 储存条件

    12 months from date of receipt / reconstitution, -20 °C as supplied

稀释度
应用 稀释度
WB 1:1000
IHC-P 1:500
IP 1:50
背景介绍
  • p90RSK, also known as RSK1 or RPS6KA1 (ribosomal protein S6 kinase alpha-1), belongs to the serine/threonine kinase family. It is a widely expressed kinase that is involved in regulating various cellular activities such as cell growth, proliferation, and survival. As a downstream effector molecule in the mitogen-activated protein kinase (MAPK) pathway, p90RSK plays a crucial role.

  • 免疫印迹

    • WB result of p90RSK Rabbit pAb
      Primary antibody: p90RSK Rabbit pAb at 1/1000 dilution
      Lane 1: HeLa whole cell lysate 20 µg
      Lane 2: MCF7 whole cell lysate 20 µg
      Lane 3: A431 whole cell lysate 20 µg
      Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 83 kDa
      Observed MW: 83 kDa

    • WB result of p90RSK Rabbit pAb
      Primary antibody: p90RSK Rabbit pAb at 1/1000 dilution
      Lane 1: RAW264.7 whole cell lysate 20 µg
      Lane 2: mouse lung lysate 20 µg
      Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 83 kDa
      Observed MW: 83 kDa

    • WB result of p90RSK Rabbit pAb
      Primary antibody: p90RSK Rabbit pAb at 1/1000 dilution
      Lane 1: PC-12 whole cell lysate 20 µg
      Lane 2: rat lung Lysate 20 µg
      Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 83 kDa
      Observed MW: 83 kDa

  • 免疫沉淀

    • p90RSK Rabbit pAb at 1/50 dilution (1 µg) immunoprecipitating p90RSK in 0.4 mg HeLa whole cell lysate.
      Western blot was performed on the immunoprecipitate using p90RSK Rabbit pAb at 1/1000 dilution.
      Secondary antibody (HRP) for IP was used at 1/1000 dilution.
      Lane 1: HeLa whole cell lysate 20 µg (Input)
      Lane 2: p90RSK Rabbit pAb IP in HeLa whole cell lysate
      Lane 3: Rabbit monoclonal IgG IP in HeLa whole cell lysate
      Predicted MW: 83 kDa 
      Observed MW: 83 kDa

  • 免疫组化

    • IHC shows positive staining in paraffin-embedded human colon. Anti-p90RSK antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human kidney. Anti-p90RSK antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human stomach. Anti-p90RSK antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human gastric cancer. Anti-p90RSK antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded mouse kidney. Anti-p90RSK antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded rat stomach. Anti-p90RSK antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.