12 months from date of receipt / reconstitution, 2 to 8 °C as supplied
| 应用 | 稀释度 |
|---|---|
| IF | 1:500 |
The CD79 molecule, comprising two polypeptide chains, mb-1 (CD79a) and B29 (CD79b), is physically associated in the B-cell membrane with immunoglobulin [PMID: 7632952]. CD79a has been reported to exhibit a high degree of linage-specificity for B-cell differentiation, with a specificity of 88% and a sensitivity of 100% [PMID: 34672246].
IF shows positive staining in paraffin-embedded human tonsil. Anti-CD79a/MB-1 (Alexa Fluor® 594 Conjugate) antibody was used at 1/500 dilution (Red) and incubated overnight at 4°C. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
IF shows positive staining in paraffin-embedded human colon. Anti-CD79a/MB-1 (Alexa Fluor® 594 Conjugate) antibody was used at 1/500 dilution (Red) and incubated overnight at 4°C. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
Negative control: IF shows negative staining in paraffin-embedded human skeletal muscle. Anti-CD79a/MB-1 (Alexa Fluor® 594 Conjugate) antibody was used at 1/500 dilution and incubated overnight at 4°C. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
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