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alpha smooth muscle Actin Recombinant Rabbit mAb (Alexa Fluor® 700 Conjugate) (S-132-63)

alpha smooth muscle Actin Recombinant Rabbit mAb (Alexa Fluor® 700 Conjugate) (S-132-63)

货号: S0B1508
价格: 1056
规格: 25μl
介绍: -
其他: -
产品规格
  • 宿主来源

    Rabbit
  • 分子别名

    SMA, Alpha-actin-2, ACTA2
  • 免疫原

    Synthetic Peptide
  • 细胞定位

    Cytoplasm, Cytoskeleton
  • Accession

    P62736
  • 克隆号

    S-132-63
  • 抗体类型

    Recombinant mAb
  • 抗体同种型

    IgG
  • 应用

    IF
  • 反应种属 ?

    Hu, Ms, Rt
  • 纯化方式

    Protein A
  • 浓度

    2 mg/ml
  • 标记

    Alexa Fluor® 700
  • 性状

    Liquid
  • 缓冲体系

    PBS, 25% Glycerol, 1% BSA, 0.3% Proclin 300
  • 储存条件

    12 months from date of receipt / reconstitution, -20 °C as supplied

稀释度
应用 稀释度
IF 1:100
背景介绍
  • α-smooth muscle actin, encoded by ACTA2, is expressed in abundance in vascular smooth muscle, comprising 50–70% of total actin, with the remainder composed of β-cytoplasmic and γ-actins. Whereas Sα-smooth muscle expression is normally restricted to smooth muscle cells, it can also be expressed in nonmuscle cells, most notably myofibroblasts that use cell traction forces to remodel extracellular matrix.

  • 免疫荧光

    • IF shows positive staining in paraffin-embedded human prostate. Anti-alpha smooth muscle Actin (Alexa Fluor® 700 Conjugate) antibody was used at 1/100 dilution (magenta) and incubated overnight at 4°C. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.

    • IF shows positive staining in paraffin-embedded human stomach. Anti-alpha smooth muscle Actin (Alexa Fluor® 700 Conjugate) antibody was used at 1/100 dilution (magenta) and incubated overnight at 4°C. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.

    • IF shows positive staining in paraffin-embedded mouse colon. Anti-alpha smooth muscle Actin (Alexa Fluor® 700 Conjugate) antibody was used at 1/100 dilution (magenta) and incubated overnight at 4°C. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.

    • IF shows positive staining in paraffin-embedded rat colon. Anti-alpha smooth muscle Actin (Alexa Fluor® 700 Conjugate) antibody was used at 1/100 dilution (magenta) and incubated overnight at 4°C. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.