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CD79a/MB-1 Recombinant Rabbit mAb (Alexa Fluor® 555 Conjugate) (S-030-18)

CD79a/MB-1 Recombinant Rabbit mAb (Alexa Fluor® 555 Conjugate) (S-030-18)

货号: S0B1504
价格: 1056
规格: 25μl
介绍: -
其他: -
产品规格
  • 宿主来源

    Rabbit
  • 分子别名

    Ig-alpha, Surface IgM-associated protein, IGA
  • 免疫原

    Synthetic Peptide
  • 细胞定位

    Cell membrane
  • Accession

    P11912
  • 克隆号

    S-030-18
  • 抗体类型

    Recombinant mAb
  • 抗体同种型

    IgG
  • 应用

    IF
  • 反应种属 ?

    Hu
  • 纯化方式

    Protein A
  • 浓度

    2 mg/ml
  • 标记

    Alexa Fluor® 555
  • 性状

    Liquid
  • 缓冲体系

    PBS, 0.1% BSA, 0.01% Proclin 300
  • 储存条件

    12 months from date of receipt / reconstitution, 2 to 8 °C as supplied

稀释度
应用 稀释度
IF 1:200
背景介绍
  • The CD79 molecule, comprising two polypeptide chains, mb-1 (CD79a) and B29 (CD79b), is physically associated in the B-cell membrane with immunoglobulin. CD79a has been reported to exhibit a high degree of linage-specificity for B-cell differentiation, with a specificity of 88% and a sensitivity of 100%.

  • 免疫荧光

    • IF shows positive staining in paraffin-embedded human tonsil. Anti-CD79a (Alexa Fluor® 555 Conjugate) antibody was used at 1/200 dilution (Red) and incubated overnight at 4°C. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.

    • IF shows positive staining in paraffin-embedded human spleen. Anti-CD79a (Alexa Fluor® 555 Conjugate) antibody was used at 1/200 dilution (Red) and incubated overnight at 4°C. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.

    • IF shows positive staining in paraffin-embedded human colon. Anti-CD79a (Alexa Fluor® 555 Conjugate) antibody was used at 1/200 dilution (Red) and incubated overnight at 4°C. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.

    • Negative control: IF shows negative staining in paraffin-embedded human skeletal muscle. Anti-CD79a (Alexa Fluor® 555 Conjugate) antibody was used at 1/200 dilution and incubated overnight at 4°C. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.