12 months from date of receipt / reconstitution, -20 °C as supplied
| 应用 | 稀释度 |
|---|---|
| WB | 1:1000-1:5000 |
IκBα (nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha) is one member of a family of cellular proteins that function to inhibit the NF-κB transcription factor. IκBα inhibits NF-κB by masking the nuclear localization signals (NLS) of NF-κB proteins and keeping them sequestered in an inactive state in the cytoplasm. In addition, IκBα blocks the ability of NF-κB transcription factors to bind to DNA, which is required for NF-κB's proper functioning.
WB result of Phospho-IκBα (Ser32/36) Rabbit mAb
Primary antibody: Phospho-IκBα (Ser32/36) Rabbit mAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: HeLa treated with Calyculin A (100ng/ml, 30min) and TNF-α (20ng/ml, 5min) whole cell lysate 20 µg
Predicted MW: 36 kDa
Observed MW: 38 kDa
(This blot was developed with high sensitivity substrate)
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