| 应用 | 稀释度 |
|---|---|
| FCM | 5 μl per million cells in 100μl volume |
CD9 belongs to the cell surface glycoprotein cross -membrane four -protein family, with four cross -membrane domains, a shorter outer domain (ECL1), and a longer extracellular domain (ECL2). It is expressed in a variety of hematopoietic cells and epithelial cells. For example, Pre B cells, B cell subset, activated T cells, basophils, eosinophils, macrophages, megakaryocytes, plasma cells, plasma cell precursors in germinal centers, and platelets. Broadcasting a series of cell processes such as cell adhesion, movement, membrane tissue and signal transfusion. The lowering of CD9 expression is related to the adverse prognosis and progress of various cancers. It is a favorable marker of gallbladder cancer, gastic GIST, malignant cortex and oral squamous cell carcinoma.
Flow cytometric analysis of CD9 expression on HCT116. Cells from the HCT116 (Human colorectal carcinoma epithelial cell, Right) or K562 (Human chronic myelogenous leukemia lymphoblast, Left) was stained with FITC Mouse IgG1, κ Isotype Control (Black line histogram) and SDT FITC Mouse Anti-Human CD9 antibody (Red line histogram) at 0.1 μg/test, cells without incubation with primary antibody and secondary antibody (Blue line histogram) was used as unlabelled control. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
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