PBS, pH7.4
12 months from date of receipt / reconstitution, 2 to 8 °C as supplied
| 应用 | 稀释度 | 推荐种属 |
|---|---|---|
| ELISA | 0.5-2 μg/ml | Rb |
| IHC-P | 10-40 μg/ml | Rb |
This antibody has been tested by ELISA to ensure minimal cross-reaction with human serum proteins.
IHC analysis of paraffin-embedded human tonsil labelling vimentin antibody (S0B2254) at 1/1000 dilution (Left) compared with PBS negative control (Right), Goat Anti-Rabbit IgG (H+L) (min X Hu Sr Prot) (S0B4011) was used as the secondary antibody, followed by a HRP Donkey anti-Goat IgG (H+L). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC analysis of paraffin-embedded human stomach labelling vimentin antibody (S0B2254) at 1/1000 dilution (Left) compared with PBS negative control (Right), Goat Anti-Rabbit IgG (H+L) (min X Hu Sr Prot) (S0B4011) was used as the secondary antibody, followed by a HRP Donkey anti-Goat IgG (H+L). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
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