50 and 25 kD in reducing condition, 150kD in non-reducing condition
-80℃, avoiding
repeated freeze-thaw cycles
ELISA analysis specificity of DM1&DM4 mAb
Coating ADC: 1μg/ml
Add anti-DM1&DM4 mAb (biotin conjugated): from 1μg/ml, 3-fold diluent, 7 points
Add SA-HRP: 1:10000
Sandwich ELISA assay
Coating anti-DM1&DM4 mAb: 5μg/ml
Sample: Trastuzumab emtansine (DM1 ADC), from 12.5ng/ml, 2-fold diluent, 7 points
Add anti human Fc HRP: 1:4000