您现在的位置： 首页   -  产品中心   -  免疫学   -   抗体
MTAP Recombinant Rabbit mAb (S-813-23)

MTAP Recombinant Rabbit mAb (S-813-23)

货号: S0B0610
价格: 260
规格: 10μl
介绍: -
其他: -
产品规格

  • 宿主来源

    Rabbit

  • 抗原名称

    RNA polymerase II CTD repeat YSPTSPS (phospho S2)

  • 分子别名

    S-methyl-5'-thioadenosine phosphorylase, 5'-methylthioadenosine phosphorylase, MTA phosphorylase, MTAPase, MSAP

  • 免疫原

    Synthetic Peptide

  • 细胞定位

    Cytoplasm, Nucleus

  • Accession

    Q13126

  • 克隆号

    S-813-23

  • 抗体类型

    Recombinant mAb

  • 抗体同种型

    IgG

  • 应用

    IHC-P, WB

  • 反应种属 ?

    Hu, Ms, Rt

  • 纯化方式

    Protein A

  • 浓度

    0.5 mg/ml

  • 标记

    Unconjugated

  • 性状

    Liquid

  • 缓冲体系

    PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300

  • 储存条件

    12 months from date of receipt / reconstitution, -20 °C as supplied

稀释度
应用 稀释度
WB 1:1000
IHC-P 1:250
背景介绍

  • S-methyl-5'-thioadenosine phosphorylase (MTAP) is an enzyme that plays a major role in polyamine metabolism and is important for the salvage of both adenine and methionine. It is responsible for the first step in this pathway, where it catalyzes the reversible phosphorylation of MTA to adenine and 5-methylthioribose-1-phosphate. This takes place after MTA is generated from S-adenosylmethionine.

  • 免疫印迹

    • WB result of MTAP Rabbit mAb 
      Primary antibody: MTAP Rabbit mAb at 1/1000 dilution
      Lane 1: A549 whole cell lysate 20 µg
      Lane 2: MCF7 whole cell lysate 20 µg
      Lane 3: HeLa whole cell lysate 20 µg
      Lane 4: HEK-293 whole cell lysate 20 µg
      Lane 5: HT-29 whole cell lysate 20 µg
      Negative control: A549 whole cell lysate ; MCF7 whole cell lysate 
      Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 31 kDa
      Observed MW: 28 kDa
      This blot was developed with high sensitivity substrate

    • WB result of MTAP Rabbit mAb 
      Primary antibody: MTAP Rabbit mAb at 1/1000 dilution
      Lane 1: RAW 264.7 whole cell lysate 20 µg
      Lane 2: NIH/3T3 whole cell lysate 20 µg
      Lane 3: mouse kidney lysate 20 µg
      Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 31 kDa
      Observed MW: 28 kDa
      This blot was developed with high sensitivity substrate

    • WB result of MTAP Rabbit mAb 
      Primary antibody: MTAP Rabbit mAb at 1/1000 dilution
      Lane 1: C6 whole cell lysate 20 µg
      Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 31 kDa
      Observed MW: 28 kDa

  • 免疫组化

    • IHC shows positive staining in paraffin-embedded human colon. Anti-MTAP antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human gastric cancer. Anti-MTAP antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.