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Phospho-Akt (Ser473) Recombinant Rabbit mAb (S-763-7)

Phospho-Akt (Ser473) Recombinant Rabbit mAb (S-763-7)

货号: S0B0596
价格: 600
规格: 25μl
介绍: -
其他: -
产品规格

  • 宿主来源

    Rabbit

  • 抗原名称

    Phospho-Akt (Ser473)

  • 免疫原

    Synthetic Peptide

  • 细胞定位

    Cytoplasm, Nucleus

  • Accession

    Q9Y243

  • 克隆号

    S-763-7

  • 抗体类型

    Recombinant mAb

  • 抗体同种型

    IgG

  • 应用

    WB, IP

  • 反应种属 ?

    Hu, Ms, Rt

  • 纯化方式

    Protein A

  • 浓度

    0.5 mg/ml

  • 标记

    Unconjugated

  • 性状

    Liquid

  • 缓冲体系

    PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300

  • 储存条件

    12 months from date of receipt / reconstitution, -20 °C as supplied

稀释度
应用 稀释度
WB 1:1000
IP 1:50
背景介绍

  • Akt, also known as Protein kinase B (PKB), is the collective name of a set of three serine/threonine-specific protein kinases that play key roles in multiple cellular processes such as glucose metabolism, apoptosis, cell proliferation, transcription, and cell migration. Once correctly positioned at the membrane via binding of PIP3, Akt can then be phosphorylated by its activating kinases, phosphoinositide-dependent kinase-1 (PDPK1 at threonine 308 in Akt1 and threonine 309 in Akt2) and the mammalian target of rapamycin complex 2 (mTORC2 at serine 473 (Akt1) and 474 (Akt2)). Activated Akt isoforms can then go on to activate or deactivate their myriad substrates (e.g. mTOR) via their kinase activity. The Akt kinases regulate cellular survival and metabolism by binding and regulating many downstream effectors, e.g. Nuclear Factor-κB, Bcl-2 family proteins, master lysosomal regulator TFEB and murine double minute 2 (MDM2).

  • 免疫印迹

    • WB result of Phospho-Akt (Ser473) Rabbit mAb
      Primary antibody: Phospho-Akt (Ser473) Rabbit mAb at 1/1000 dilution
      Lane 1: untreated Jurkat whole cell lysate 20 µg
      Lane 2: Jurkat treated with Calyculin A (100 nM, 30 mins) whole cell lysate 20 µg
      Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 56 kDa
      Observed MW: 60 kDa

    • WB result of Phospho-Akt (Ser473) Rabbit mAb
      Primary antibody: Phospho-Akt (Ser473) Rabbit mAb at 1/1000 dilution
      Lane 1: untreated NIH/3T3 whole cell lysate 20 µg
      Lane 2: NIH/3T3 treated with Calyculin A (100 nM, 30 mins) whole cell lysate 20 µg
      Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 56 kDa
      Observed MW: 60 kDa

    • WB result of Phospho-Akt (Ser473) Rabbit mAb
      Primary antibody: Phospho-Akt (Ser473) Rabbit mAb at 1/1000 dilution
      Lane 1: untreated C6 whole cell lysate 20 µg
      Lane 2: C6 treated with Calyculin A (100 nM, 30 mins) whole cell lysate 20 µg
      Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 56 kDa
      Observed MW: 60 kDa

  • 免疫沉淀

    • Phospho-Akt (Ser473) Rabbit mAb at 1/50 dilution (1 µg) immunoprecipitating Phospho-Akt (Ser473) in 0.4 mg Jurkat treated with Calyculin A (100 nM, 30 mins) whole cell lysate.
      Western blot was performed on the immunoprecipitate using Phospho-Akt (Ser473) Rabbit mAb at 1/1000 dilution.
      Secondary antibody (HRP) for IP was used at 1/1000 dilution.
      Lane 1: Jurkat treated with Calyculin A (100 nM, 30 mins) whole cell lysate 20 µg (Input)
      Lane 2: Phospho-Akt (Ser473) Rabbit mAb IP in Jurkat treated with Calyculin A (100 nM, 30 mins) whole cell lysate
      Lane 3: Rabbit monoclonal IgG IP in Jurkat treated with Calyculin A (100 nM, 30 mins) whole cell lysate
      Predicted MW: 56 kDa
      Observed MW: 60 kDa
      This blot was developed with high sensitivity substrate

  • 斑点杂交

    • Dot blot result of Phospho-Akt (Ser473) Rabbit mAb
      Lane 1: Phospho-Akt (Ser473) peptide
      Lane 2: Akt unmodified peptide
      Primary antibody: Phospho-Akt (Ser473) Rabbit mAb at 1/1000 dilution
      Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution