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α-tubulin Mouse mAb (S-364-23)

α-tubulin Mouse mAb (S-364-23)

货号: S0B0578
价格: 600
规格: 25μl
介绍: -
其他: -
产品规格
  • 宿主来源

    Mouse
  • 抗原名称

    α-tubulin
  • 分子别名

    Tubulin alpha-4A chain, Alpha-tubulin 1
  • 免疫原

    Synthetic Peptide
  • 细胞定位

    Cytoskeleton, Cytoplasm
  • Accession

    P68366
  • 克隆号

    S-364-23
  • 抗体类型

    Mouse mAb
  • 抗体同种型

    IgG1,k
  • 应用

    ICFCM, IHC-P, ICC, WB
  • 反应种属 ?

    PIG, Zf, Hu, Ms, Rt
  • 预测反应种属
    (反应种属缩写表)

    Bv, SeUr, Pg, Lob, Cz, Pl, Fs, Dr, Ar, Xe, Hm, Av
  • 纯化方式

    Protein G
  • 浓度

    2 mg/ml
  • 标记

    Unconjugated
  • 性状

    Liquid
  • 缓冲体系

    PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
  • 储存条件

    12 months from date of receipt / reconstitution, -20 °C as supplied

稀释度
应用 稀释度
WB 1:1000~1:5000
IHC-P 1:500
ICC 1:1000
ICFCM 1:2000
背景介绍
  • Tubulin is the major constituent of microtubules, a cylinder consisting of laterally associated linear protofilaments composed of alpha- and beta-tubulin heterodimers. Tubulin α- and β-subunits have molecular weights of ~ 50 kDa and are 36%–42% identical and 63% homologous. Both tubulin subunits bind guanine nucleotides. The binding to α-tubulin at the N-site is nonexchangeable, while the binding to β-tubulin at the E-site is exchangeable. Nucleotide in microtubules does not exchange with the solution, except for terminal subunits at microtubule ends.

  • 免疫印迹

    • WB result of α-tubulin Mouse mAb
      Primary antibody: α-tubulin Mouse mAb at 1/1000 dilution
      Lane 1: HeLa whole cell lysate 20 µg
      Secondary antibody: Goat Anti-Mouse IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 55 kDa
      Observed MW: 52 kDa

    • WB result of α-tubulin Mouse mAb
      Primary antibody: α-tubulin Mouse mAb at 1/1000 dilution
      Lane 1: NIH/3T3 whole cell lysate 20 µg
      Secondary antibody: Goat Anti-Mouse IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 55 kDa
      Observed MW: 52 kDa

    • WB result of α-tubulin Mouse mAb
      Primary antibody: α-tubulin Mouse mAb at 1/1000 dilution
      Lane 1: C6 whole cell lysate 20 µg
      Secondary antibody: Goat Anti-Mouse IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 55 kDa
      Observed MW: 52 kDa

    • WB result of α-tubulin Mouse mAb
      Primary antibody: α-tubulin Mouse mAb at 1/5000 dilution
      Lane 1: pig heart lysate 20 µg
      Secondary antibody: Goat Anti-Mouse IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 55 kDa
      Observed MW: 52 kDa
      (This blot was developed with high sensitivity substrate)

    • WB result of α-tubulin Mouse mAb 
      Primary antibody : α-tubulin Mouse mAb at 1/1000 dilution
      Lane 1 : Zebra fish lysate 20 µg
      Secondary antibody: Goat Anti-Mouse IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 55 kDa
      Observed MW: 52 kDa

  • 流式分析

    • Flow cytometric analysis of 4% PFA fixed 90% methanol permeabilized HeLa (Human cervix adenocarcinoma epithelial cell) cells labelling α-tubulin antibody at 1/2000 dilution (0.1 μg) / (Red) compared with a Mouse monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti - Mouse IgG Alexa Fluor® 488 was used as the secondary antibody.

  • 免疫组化

    • IHC shows positive staining in paraffin-embedded human kidney. Anti-α-tubulin antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human colon cancer. Anti-α-tubulin antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human endometrial cancer. Anti-α-tubulin antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded mouse cerebral cortex. Anti-α-tubulin antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded rat cerebral cortex. Anti-α-tubulin antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

  • 免疫细胞化学

    • ICC shows positive staining in HeLa cells. Anti-α-tubulin antibody was used at 1/1000 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Mouse IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue).