PBS pH7.4, containing no preservative
2 to 8 °C for 2 weeks under sterile conditions;
-20 °C for 3 months under sterile conditions;
-80 °C for 24 months under sterile conditions.
Please avoid repeated freeze-thaw cycles.
应用 | 稀释度 |
---|---|
WB | 1:1000 |
This antibody recognizes the same epitope as clone 9D9.
Cytotoxic T-lymphocyte associated protein 4, (CTLA-4) also known as CD152 (cluster of differentiation 152), is a protein receptor that functions as an immune checkpoint and downregulates immune responses. CTLA-4 is constitutively expressed in regulatory T cells but only upregulated in conventional T cells after activation – a phenomenon which is particularly notable in cancers. CTLA-4 may also function via modulation of cell motility and/or signaling through PI3 kinase.
Animal species and strain: BALB/c
Cell line: EMT6
Animal species and strain: BALB/c
Cell line: EMT6
WB result of Invivo CTLA-4 (CD152) Mouse mAb
Primary antibody: Invivo CTLA-4 (CD152) Mouse mAb at 1/1000 dilution
Lane 1: reduced purified mouse CTLA-4 with histidine tag at C-terminus 1 µg
Lane 2: reduced purified mouse CTLA-4 with histidine tag at C-terminus 0.5 µg
Secondary antibody: Goat Anti-Mouse IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 25~30 kDa
Observed MW: 20~30 kDa
Anti-His antibody Immobilized on CM5 Chip captured CD152 His Tag, Mouse, can bind CTLA-4 (CD152) Mouse mAb (9D9) with an affinity constant of 2.315 nM as determined in SPR assay.