12 months from date of receipt / reconstitution, 2 to 8 °C as supplied
| 应用 | 稀释度 |
|---|---|
| ICC | 1:500 |
| IF | 1:500 |
ICC shows positive staining in HeLa cells. Anti-alpha smooth muscle Actin antibody (S0B0102) was used at 1/100 dilution (Red) and incubated overnight at 4°C. Goat anti-Mouse IgG (H+L) (Alexa Fluor® 594 Conjugate) was used as secondary antibody at 1/500 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue).
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