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MUM1/IRF4 Recombinant Rabbit mAb (S-654-33)

MUM1/IRF4 Recombinant Rabbit mAb (S-654-33)

货号: S0B0548
价格: 600
规格: 25μl
介绍: -
其他: -
产品规格
  • 宿主来源

    Rabbit
  • 分子别名

    Interferon regulatory factor 4, Lymphocyte-specific interferon regulatory factor (LSIRF), Multiple myeloma oncogene 1, NF-EM5
  • 免疫原

    Recombinant Protein
  • 细胞定位

    Nucleus
  • Accession

    Q15306
  • 克隆号

    S-654-33
  • 抗体类型

    Recombinant mAb
  • 抗体同种型

    IgG
  • 应用

    IHC-P, WB
  • 反应种属 ?

    Hu
  • 纯化方式

    Protein A
  • 浓度

    0.5 mg/ml
  • 标记

    Unconjugated
  • 性状

    Liquid
  • 缓冲体系

    PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
  • 储存条件

    12 months from date of receipt / reconstitution, -20 °C as supplied

稀释度
应用 稀释度
WB 1:1000
IHC-P 1:500
背景介绍
  • Interferon regulatory factor 4 (IRF4) also known as MUM1 is a protein that in humans is encoded by the IRF4 gene. IRF4 functions as a key regulatory transcription factor in the development of human immune cells. The expression of IRF4 is essential for the differentiation of T lymphocytes and B lymphocytes as well as certain myeloid cells. Dysregulation of the IRF4 gene can result in IRF4 functioning either as an oncogene or a tumor-suppressor, depending on the context of the modification.

  • 免疫印迹

    • WB result of MUM1/IRF4 Rabbit mAb
      Primary antibody: MUM1/IRF4 Rabbit mAb at 1/1000 dilution
      Lane 1: HuT 78 whole cell lysate 20 µg
      Lane 2: Ramos whole cell lysate 20 µg
      Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 52 kDa
      Observed MW: 52 kDa

  • 免疫组化

    • IHC shows positive staining in paraffin-embedded classical Hodgkin's lymphoma. Anti-MUM1/IRF4 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human colon cancer. Anti-MUM1/IRF4 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human endometrial cancer. Anti-MUM1/IRF4 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human colon. Anti-MUM1/IRF4 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human tonsil. Anti-MUM1/IRF4 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.